AbcamBiochemicals

Dynamin Inhibitors: MiTMAB™ Series Kit (ab120473)

Overview

  • Product nameDynamin Inhibitors: MiTMAB™ Series Kit
  • SpecificityConvenient kit of dynamin inhibitors from the MiTMAB™ chemical series. The inhibitors target dynamin at the lipid binding (PH) domain and inhibit dynamin and endocytosis in enzymatic and cell based assays. They are based on the same chemical scaffold. A negative control compound is also included. Kit contains 1 mg of each of MiTMAB™ (ab120466), OcTMAB™ (ab120467), and Pro-Mystyric Acid™ (ab120476).
  • Product overview

    MiTMABTM chemical series collection


    Convenient kit of dynamin inhibitors from the MiTMAB™ chemical series. The inhibitors target dynamin at the lipid binding (PH) domain and inhibit dynamin and endocytosis in enzymatic and cell based assays. They are based on the same chemical scaffold. A negative control compound is also included. Kit contains 1 mg of each of MiTMAB™ (ab120466), OcTMAB™ (ab120467), and Pro-Mystyric Acid™ (ab120476).

    Pro-Myristic acid is an in vitro inhibitor of dynamin. Although cell permeable, it is rapidly broken down by cellular esterases to release intracellular myristic acid, which is not a dynamin inhibitor. It can therefore be used as a negative control in cell-based studies.


    TargetDescription
    PH lipid binding site

    MiTMAB™:


    Cell permeable dynamin I and dynamin II inhibitor

    PH lipid binding site

    OcTMAB™:


    Cell permeable dynamin I and dynamin II inhibitor

    PH lipid binding site

    Pro-Myristic Acid:


    Negative control for MiTMAB™ and OcTMAB™

     

    Convenient kit of dynamin inhibitors from the MiTMAB™ chemical series. The inhibitors target dynamin at the lipid binding (PH) domain and inhibit dynamin and endocytosis in enzymatic and cell based assays. They are based on the same chemical scaffold. A negative control compound is also included. Kit contains 1 mg of each of MiTMAB™ ab120476.

  • Notes

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. MiTMAB™ and OcTMAB™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120473 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Dynamin Inhibitors: MiTMAB™ Series Kit images

    • ab66705 staining PAI1 in HeLa cells treated with OcTMAB™ (ab120467), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of OcTMAB™, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120467 (OcTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    • ab66705 staining PAI1 in HeLa cells treated with MiTMAB™ (ab120466), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of MiTMAB™, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120466 (MiTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Dynamin Inhibitors: MiTMAB™ Series Kit (ab120473)

    ab120473 has not yet been referenced specifically in any publications.

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