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Product name
Anti-Dynein light chain antibody
See all Dynein light chain products (4) ...
Description
Chicken polyclonal to Dynein light chain
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Full length native protein (purified) (Human): Dynein light chain
Positive control
E coli-derived fusion protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Azide free.
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Dynein
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Western blot - Dynein light chain antibody (ab37464)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/2000. Predicted molecular weight: 12 kDa.
We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity.
Sequence similarities
Belongs to the dynein light chain family.
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme.
Target information above from: UniProt accessionO96015
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- anti-DNAL4 antibody
- axonemal antibody
- Bithoraxoid like protein antibody
see all
Database links
- Entrez Gene: 10126 Human
- Entrez Gene: 54152 Mouse
- Entrez Gene: 300078 Rat
- Omim: 610565 Human
- SwissProt: O96015 Human
- SwissProt: Q9DCM4 Mouse
- Unigene: 632766 Human
- Unigene: 288159 Mouse
see all
Anti-Dynein light chain antibody images:
Western blot - Dynein light chain antibody (ab37464)
Anti-Dynein light chain antibody (ab37464) at 1/2000 dilution + E coli derived fusion protein
Secondary
Goat anti IgY HRP at 1/1000 dilution
Predicted band size : 12 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Difference between predicted and actual band size is due to the fact that this western blot is representing a fusion protein (not the native form).
References for Anti-Dynein light chain antibody (ab37464)
ab37464 has not yet been referenced specifically in any publications.
Publishing research using ab37464? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"