E Cadherin Mouse ELISA Kit (ab100678)
- Product nameE Cadherin Mouse ELISA KitSee all E Cadherin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 6 pg/ml
- Range4.12 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 114.7 103% - 123% Serum 78.78 69% - 88% Plasma 88.93 76% - 95%
- Species reactivityReacts with: Mouse
- Product overview
ab100678 E Cadherin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse E Cadherin in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Mouse E Cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and E Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse E Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of E Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml Detection Antibody E Cadherin: biotinylated anti-mouse E Cadherin 2 vials E Cadherin Microplate (12 strips x 8 wells) coated with anti-mouse E Cadherin 1 unit Mouse E Cadherin Standard (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- Arc 1Cadherin 1cadherin 1 type 1 E-cadherin
- Cadherin1CAM 120/80CD 234CD324CD324 antigenCDH1CDHEECAD Epithelial cadherinepithelial calcium dependant adhesion proteinLCAMLiver cell adhesion moleculeUVOUvomorulin
Our Abpromise guarantee covers the use of ab100678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for E Cadherin Mouse ELISA Kit (ab100678)
ab100678 has not yet been referenced specifically in any publications.