Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)

Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.

Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)

ab15148 staining human MCF10A cells by ICC/IF.  Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C.  The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C.  An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Dr Mauricio Pinto

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)

ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

This image is a courtesy of Anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)

ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37ºC; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4ºC for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)

ab15148 staining E cadherin in Human AGS Gastric carcinoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 23ºC. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23ºC. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Frozen sections) - E Cadherin antibody (ab15148)

ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C.  An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary.

This image is courtesy of an anonymous Abreview.

Western blot - E Cadherin antibody (ab15148)

All lanes : Anti-E Cadherin antibody (ab15148) at 1/500 dilution (for 16 hours at 4°C)

Lane 1 : Human OE33 cell - whole cell lysate
Lane 2 : Human OE33 cell - whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/1000 dilution
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 120 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 minute

Blocking Step: 5% BSA fro 1 hour at 23°C

This image is courtesy of an anonymous Abreview