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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Overview
Product name
Anti-E Cadherin antibody
See all E Cadherin products (23) ...
Description
Rabbit polyclonal to E Cadherin
Specificity
We have data to indicate that this antibody may not cross react with Chicken. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
WB, ICC, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human, Pig
Immunogen
Recombinant fragment: IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASANWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDNQNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI , corresponding to amino acids 600-707 of Human E Cadherin.
IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASA NWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDN QNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI
Positive control
Skin
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: Sodium Azide
Constituents: 10mM PBS, BSA. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
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Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab15148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 120 kDa.
ICC: Use at an assay dependent dilution.
IHC-P: 1/30(for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC-Fr: 1/50
Target
Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Tissue specificity
Non-neural epithelial tissues.
Involvement in disease
Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Sequence similarities
Contains 5 cadherin domains.
Post-translational
modifications
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
Cellular localization
Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Target information above from: UniProt accessionP12830
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Arc 1 antibody
- CADH1_HUMAN antibody
- Cadherin 1 antibody
see all
Database links
- Entrez Gene: 999 Human
- Entrez Gene: 100048953 Pig
- Omim: 192090 Human
- SwissProt: P12830 Human
- Unigene: 461086 Human
Anti-E Cadherin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.
Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)
ab15148 staining human MCF10A cells by ICC/IF. Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C. The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr Mauricio Pinto
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
This image is a courtesy of Anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37°C; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4°C for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)
ab15148 staining E cadherin in Human AGS Gastric carcinoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Frozen sections) - E Cadherin antibody (ab15148)
ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C. An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary.
This image is courtesy of an anonymous Abreview.
Western blot - E Cadherin antibody (ab15148)
All lanes : Anti-E Cadherin antibody (ab15148) at 1/500 dilution (for 16 hours at 4°C)
Lane 1 : Human OE33 cell - whole cell lysate
Lane 2 : Human OE33 cell - whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Blocking Step: 5% BSA fro 1 hour at 23°C
This image is courtesy of an anonymous Abreview
References for Anti-E Cadherin antibody (ab15148)
This product has been referenced in:
- Butler TMet al. Integrin-linked kinase can facilitate syncytialization and hormonal differentiation of the human trophoblast-derived BeWo cell line. Reprod Biol Endocrinol 7:51 (2009). ICC/IF; Human.Read more (PubMed: 19463178) »
- Hughes JNet al. A novel role for gamma-secretase in the formation of primitive streak-like intermediates from ES cells in culture. Stem Cells 27:2941-51 (2009).Read more (PubMed: 19750540) »
See all 4 publications for this product
Publishing research using ab15148? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"