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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
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Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
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Product Name
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E Cadherin antibody
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See all E Cadherin antibodies (21)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to E Cadherin
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Immunogen
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Recombinant fragment: IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASANWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDNQNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI , corresponding to amino acids 600-707 of Human E Cadherin.
BLAST 'IFFCERNPKP QVINIIDAD L PPNTSPFT AE LTHGASA NWT IQYNDP TQES IILKP KMALE VGDY KINLKL MDN QNKDQVT TL EVSVCDCE G AAGVCRKAQ ' with  or 
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Reacts with
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Hu, Pig
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Does not react with
(species key)
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Chk
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Tested applications
(see key)
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ICC, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependent dilution.
IHC-P: 1/30. for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
IHC-Fr: 1/50. (Abreview: 18816)
WB: 1/500. Detects a band of approximately 120 kDa. (Abreview: 20303)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Skin
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Cellular localization
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Cell membrane; cell-cell junction; single-pass type I membrane protein.
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Research areas
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Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
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Relevance
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One of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: Sodium Azide
Constituents: 10mM PBS, BSA. pH 7.4
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E Cadherin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E Cadherin antibody images, references, products related to ab15148 and other tools.
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E Cadherin antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.
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Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)
ab15148 staining human MCF10A cells by ICC/IF. Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C. The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody. This image is courtesy of an Abreview submitted by Dr Mauricio Pinto
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody. This image is a courtesy of Anonymous Abreview
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37°C; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4°C for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)
ab15148 staining E cadherin in Human AGS Gastric carcinoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Frozen sections) - E Cadherin antibody (ab15148)
ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C. An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary. This image is courtesy of an anonymous Abreview.
See Abreview
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References for E Cadherin antibody (ab15148)
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This product has been used in: (two most recent references)
Butler TM et al. Integrin-linked kinase can facilitate syncytialization and hormonal differentiation of the human trophoblast-derived BeWo cell line. Reprod Biol Endocrinol 7:51 (2009). ICC/IF; Human. PubMed: 19463178
Hughes JN et al. A novel role for gamma-secretase in the formation of primitive streak-like intermediates from ES cells in culture. Stem Cells 27:2941-51 (2009). PubMed: 19750540
See all 4 publication references for this product.
If you publish research using ab15148 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to E Cadherin
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E Cadherin antibody - more information
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