|
|
|
|
|
Product Name
|
E. coli K99 pilus antibody [K99 12-444.5]
|
| |
See all E. coli K99 pilus antibodies (2)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [K99 12-444.5] to E. coli K99 pilus
|
|
Immunogen
|
Purified E. coli K99 Pili.
|
|
Reacts with
(species key)
|
E coli
|
|
Specificity
|
These antibodies do not cross-react with K99-negative E. coli, E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.
|
|
Tested applications
(see key)
|
ELISA
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Dilute in PBS or medium which is identical to that used in the assay system.
These antibody may be used in an ELISA to detect and quantitate K99-positive E. coli.
ELISA titre is 1:16,000 (indirect assay against E. coli K99 pili on the solid phase. Titres represent endpoint dilutions that achieve absorbance values greater than 0.1).
|
|
Cellular localization
|
Cell Membrane
|
|
Research areas
|
Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Escherichia coli
|
|
Relevance
|
K99 pilus antigen is frequently associated with enterotoxigenic strains of E. coli isolated from the intestine of calves, sheep and pigs. Pilus antigens have been shown to be the main attachment mechanism whereby EPEC attach to and colonize the small intestine of neonatal animals. Any mechanism which will interfere with this attachment and colonization process is capable of reducing the intestinal colonization, diarrhoea and mortality associated with K99 EPEC infections.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window SwissProt | | P18103 (Escherichia coli )
|
|
|
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
K99 12-444.5
|
|
Isotype
|
IgG1
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
PBS, pH7.2
|
|
Form
|
Liquid
|
|
Concentration
|
1.100 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E. coli K99 pilus antibody [K99 12-444.5] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to E. coli K99 pilus
|
| |
E. coli K99 pilus antibody [K99 12-444.5] - more information
|
Customer reviews (feedback) regarding E. coli K99 pilus antibody [K99 12-444.5] |
Customer FAQs regarding E. coli K99 pilus antibody [K99 12-444.5] |
Protocols for E. coli K99 pilus antibody [K99 12-444.5] |
Price and availability of products related to E. coli K99 pilus antibody [K99 12-444.5] |
| |
E. coli K99 pilus antibody [K99 12-444.5] - related products:
|
|
|
| |
E. coli K99 pilus antibody [K99 12-444.5] - other tools:
|
Contact Abcam with a Technical Enquiry about E. coli K99 pilus antibody [K99 12-444.5] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
