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Anti-E. coli K99 pilus antibody [K99 12-444.5] (ab1038)

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Overview

Product name

Anti-E. coli K99 pilus antibody [K99 12-444.5]
See all E. coli K99 pilus products (2) ...

Description

Mouse monoclonal [K99 12-444.5] to E. coli K99 pilus

Specificity

These antibodies do not cross-react with K99-negative E. coli, E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.

Tested applications

ELISAmore details

Cross reactivity

Reacts with

Escherichia coli

Immunogen

Purified E. coli K99 Pili.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, pH7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

K99 12-444.5

Isotype

IgG1

Light chain type

unknown

Research areas

Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Escherichia coli

Applications

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Our Abpromise guarantee covers the use of ab1038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

Application notes

Dilute in PBS or medium which is identical to that used in the assay system.
These antibody may be used in an ELISA to detect and quantitate K99-positive E. coli.
ELISA titre is 1:16,000 (indirect assay against E. coli K99 pili on the solid phase. Titres represent endpoint dilutions that achieve absorbance values greater than 0.1).

Target

Relevance

K99 pilus antigen is frequently associated with enterotoxigenic strains of E. coli isolated from the intestine of calves, sheep and pigs. Pilus antigens have been shown to be the main attachment mechanism whereby EPEC attach to and colonize the small intestine of neonatal animals. Any mechanism which will interfere with this attachment and colonization process is capable of reducing the intestinal colonization, diarrhoea and mortality associated with K99 EPEC infections.

Cellular localization

Cell Membrane

Alternative names

  • E.coli K99 protein antibody
  • Escherichia coli K99 pilus antibody
  • FANC antibody
  • K99 fimbrial protein antibody
  • K99 pilus antibody
see all

Database links

References for Anti-E. coli K99 pilus antibody [K99 12-444.5] (ab1038)

ab1038 has not yet been referenced specifically in any publications.

Publishing research using ab1038? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"