Recombinant E. coli RecA protein (Active) (ab174091)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE, Electron Microscopy
Description
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Product name
Recombinant E. coli RecA protein (Active)
See all RecA proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab174091 was over-expressed as a recombinant protein and highly purified by several steps of chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDI ALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHAL DPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAAL TPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKI GVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKN KIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIG QGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETN EDF -
Predicted molecular weight
36 kDa -
Amino acids
1 to 353
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Description
Recombinant E. coli RecA protein (Active)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Electron Microscopy
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 0.32% Tris-HCl buffer, 0.03% EDTA, 1.1% Potassium chloride, 0.02% DTT
General Info
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Alternative names
- DNA strand exchange and recombination protein with protease and nuclease activity
- lexB
- Protein recA
see all -
Relevance
RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. -
Cellular localization
Cytoplasmic
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab174091 has been referenced in 1 publication.
- Horii T et al. Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein. Cell 27:515-22 (1981). Functional Studies ; Escherichia coli . PubMed: 6101204