Recombinant E. coli hchA protein (ab106879)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant E. coli hchA protein -
Purity
> 95 % SDS-PAGE.
ab106879 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MGSSHHHHHHSSGLVPRGSHMTVQTSKNPQVDIAEDNAFFPSEYSLSQYT SPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLL PLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNP KKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRF VISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWY FGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQEMLAA YAG -
Predicted molecular weight
33 kDa including tags -
Amino acids
1 to 283 -
Tags
His tag N-Terminus
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-
Description
Recombinant E. coli hchA protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab106879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
General Info
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Alternative names
- Chaperone protein hchA
- EcHsp31
- ECK1963
see all -
Function
Uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. It rapidly releases them once stress has abated. -
Sequence similarities
Belongs to the peptidase C56 family. HchA subfamily. -
Domain
Consists of a large A domain and a smaller P domain connected by a linker. The thermally induced motion of the flexible linker-loop region leads to the uncovering of a high-affinity substrate-binding site that is essential to capture nonnative proteins at high temperatures. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab106879 has not yet been referenced specifically in any publications.