Overview
Product nameE. coli serine protease inhibitor proteinSee all E. coli serine protease inhibitor proteins and peptides ...
Protein descriptionRecombinant full length E. coli serine protease inhibitor (amino acids 21-162) with an N terminal His tag; 163 amino acids with tag, MWt 18.3 kDa (NP_416713).
Uniprot accessionP23827
Molecular weight18.300kDa inclusive of tags
Protein length142 amino acids
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab103303 was purified using conventional chromatography techniques.
Mass spectrometryMALDI-TOF
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 10% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
Concentration information loading...
SequenceMGSSHHHHHHSSGLVPRGSHMAESVQPLEKIAPYPQAEKG MKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKL ENKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTA YLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNA VVR
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab103303
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| MS |
MS: Use at an assay dependent dilution. |
Protein info
-
Alternative names
ECK2201EcotinEcotin, a serine protease inhibitor
see all
RelevanceE. coli serine protease inhibitor (Ecotin) inhibits pancreatic serine proteases: inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity.
Cellular localizationPeriplasm.
E. coli serine protease inhibitor protein images
References for E. coli serine protease inhibitor protein (ab103303)
ab103303
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - E. coli serine protease inhibitor protein (ab103303)15% SDS-PAGE analysis of 3µg ab103303.