E-selectin Monkey ELISA Kit (with color giving dyes) (ab119523)
Overview
- Product nameE-selectin Monkey ELISA Kit (with color giving dyes)
- Precision
Intra-assay Sample n Mean SD CV% Overall < 5% - Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity0.4 U/ml
- Range1.9 U/ml - 60 U/ml
- Recovery
85 %
- Species reactivityReacts with: Monkey
- Product overview
ab119523 E-selectin Monkey ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sE-selectin. ab119523 is for research use only and is not for diagnostic or therapeutic procedures.
Endothelial Leukocyte Adhesion Molecule-1 belongs to the selectin family of adhesion molecules. Together with L-selectin and P-selectin, E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells.
Molecular structure: The extracellular part of all selectins consists of an aminoterminal c-type lectin domain which specifically binds to carbohydrate ligands. This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats. The transmembrane portion of the molecule is followed by a short cytoplasmic tail.
Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. Together with P-selectin, E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of still resting leukocytes to the endothelium. This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators. In contrast to P-selectin, E-selectin is maximally expressed 2-4 hours after cell activation. Within the next 24-48 hours E-selectin is again eliminated from the cytoplasmic membrane by shedding into the circulation.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Adhesive Films 2 units Assay Buffer Concentrate (20x) 1 x 5ml Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml HRP-Conjugate anti-monkey sE-selectin monoclonal antibody 1 x 150µl Monkey sE-selectin Standard 2 vials Monoclonal anti-monkey sE-selectin-coated microwell plate 1 unit Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml -
Research Areas
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains.
Target information above from: UniProt accession
P16581
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD62 antigen-like family member ECD62EE-selectin
- ELAMELAM-1ELAM1Endothelial adhesion molecule 1Endothelial leukocyte adhesion molecule 1ESELLECAM2Leukocyte-endothelial cell adhesion molecule 2LYAM2_HUMANSeleSelectin E
see all
Applications
Our Abpromise guarantee covers the use of ab119523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
E-selectin Monkey ELISA Kit (with color giving dyes) images
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Sandwich ELISA - E-selectin Monkey ELISA Kit (with color giving dyes) (ab119523)Representative standard curve using ab119523.
Protocols
References for E-selectin Monkey ELISA Kit (with color giving dyes) (ab119523)
ab119523 has not yet been referenced specifically in any publications.
