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Western blot - E2F1 antibody [KH129] (ab11950)
![Western blot - E2F1 antibody [KH129] (ab11950)](/ps/datasheet/images/11950.jpg)
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Product Name
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E2F1 antibody [KH129]
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See all E2F1 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [KH129] to E2F1
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Immunogen
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Full-length protein corresponding to human E2F1.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. A band of approximately 60 kDa corresponding to E2F1 should be detected in Western blot analysis. An uncharacterized band of lower molecular weight may also be revealed under similar conditions. For optimal results, primary antibody incubations should be performed at 4°C.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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K-562 nuclear extract.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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KH129
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Isotype
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IgG1
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.2% gelatin, 0.01% thimerosal and 0.1% sodium azide
Constituents: PBS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F1 antibody [KH129] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F1 antibody [KH129] images, references, products related to ab11950 and other tools.
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E2F1 antibody [KH129] images:
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 Western blot - E2F1 antibody [KH129] (ab11950)
Predicted band size : 60 kDa
Detection of E2F-1 by Western blot analysis. The presence of E2F-1 in nuclear extracts derived from K-562 cells (TPA) is confirmed by the detection of a signal corresponding to a protein of approximately 60 kDa using ab11950 at a 1/1000 dilution.
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Search PubMed (MEDLINE) for references to E2F1
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E2F1 antibody [KH129] - more information
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Customer reviews (feedback) regarding E2F1 antibody [KH129] |
Customer FAQs regarding E2F1 antibody [KH129] |
Protocols for E2F1 antibody [KH129] |
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E2F1 antibody [KH129] - related products:
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E2F1 antibody [KH129] - other tools:
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Contact Abcam with a Technical Enquiry about E2F1 antibody [KH129] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - E2F1 antibody [KH129] (ab11950)](/index.html?datasheet=11950#11950.jpg)
