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- Secondary antibodies
- Lysates
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Product name
Anti-E2F1 antibody [KH95] (Biotin)
See all E2F1 products (20) ...
Description
Mouse monoclonal [KH95] to E2F1 (Biotin)
Conjugation
Biotin
Specificity
We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
WB, IP, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant Human E2F1 protein.
Epitope
aa 342-386
Positive control
Mad109 or HeLa cells. Tonsil or breast carcinomas.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
KH95
Isotype
IgG2a
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
IHC-P
ICC/IF
Application notes
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
IP: Use at 2µg/mg of lysate. Native and denatured.
WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similarities
Belongs to the E2F/DP family.
Post-translational
modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ01094
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 1 antibody
- E2F transcription factor 1 antibody
- E2F-1 antibody
see all
Database links
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- Unigene: 654393 Human
- Unigene: 18036 Mouse
References for Anti-E2F1 antibody [KH95] (Biotin) (ab79807)
This product has been referenced in:
- Helin K et al. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Genes Dev 7:1850-61 (1993).Read more (PubMed: 8405995) »
See 1 publication for this product
Publishing research using ab79807? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"