![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 antibody [KH95] (ab4070) image](#ab4070_1.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F1 antibody [KH95] - ChIP Grade
See all E2F1 products (20) ...
Description
Mouse monoclonal [KH95] to E2F1 - ChIP Grade
Specificity
Protein of 60 kDa.
Tested applications
ChIP, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length protein (Human).
Positive control
Tonsil and Breast carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Ascites
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
KH95
Isotype
IgG2a
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 antibody [KH95] (ab4070)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 antibody [KH95] (ab4070) image](/ps/datasheet/Images/4/ab4070/ab4070_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab4070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent concentration. (PubMed: 21518729)
IHC-P: 1/25 - 1/50.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similarities
Belongs to the E2F/DP family.
Post-translational
modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ01094
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 1 antibody
- E2F transcription factor 1 antibody
- E2F-1 antibody
see all
Database links
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Entrez Gene: 399489 Rat
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- SwissProt: O09139 Rat
- Unigene: 654393 Human
see all
Anti-E2F1 antibody [KH95] - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 antibody [KH95] (ab4070)
ab4070 staining human tonsil by IHC-P.
References for Anti-E2F1 antibody [KH95] - ChIP Grade (ab4070)
This product has been referenced in:
- Millour Jet al. ATM and p53 Regulate FOXM1 Expression via E2F in Breast Cancer Epirubicin Treatment and Resistance. Mol Cancer Ther 10:1046-58 (2011). ChIP; Human.Read more (PubMed: 21518729) »
See 1 publication for this product
Publishing research using ab4070? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"