E2F1 antibody [KH95] (ab4070)

Product Name 

E2F1 antibody [KH95]

Product type 

Primary antibodies

Description 

Mouse monoclonal [KH95] to E2F1

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Protein of 60 kDa.

Tested applications 
(see key)

IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Immunohistochemistry: 1/25 - 1/50 for 30mins at RT (ABC method).
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.

Positive control 
(see definition)

Tonsil and Breast carcinoma.

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

KH95

Isotype 

IgG2a

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Store at +4°C.

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