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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F1 antibody
See all E2F1 products (20) ...
Description
Mouse monoclonal to E2F1
Conjugation notes
-
Specificity
This antibody recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Fusion protein containing the region encoding the transactivation and retinoblastoma binding domain (amino acids 332-390) of E2F-1 expressed in E. coli.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Phosphate buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
1 mg/ml of antibody purified from hybridoma cell culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE. Prepared in 10mM PBS (pH 7.4).
Clonality
Monoclonal
Myeloma
NS1
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 47 kDa.
IP: Use at an assay dependent dilution.
Target
Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similarities
Belongs to the E2F/DP family.
Post-translational
modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ01094
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 1 antibody
- E2F transcription factor 1 antibody
- E2F-1 antibody
see all
Database links
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
References for Anti-E2F1 antibody (ab100)
ab100 has not yet been referenced specifically in any publications.
Publishing research using ab100? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"