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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Anti-E2F1 (phospho S364) antibody (ab5391)
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Secondary antibodies
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - E2F1 (phospho S364) antibody (ab5391)
All lanes : Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution
Lane 1 : CRE (mouse) cells transfected with human E2F1
Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin
Lane 3 : NiH3T3 (mouse) cell lysate
Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin
Lane 5 : MDA-231 (human breast cancer) cell lysate
Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin
Secondary
HRP conjugated Goat-anti-Rabbit IgG MX10
Predicted band size : 47 kDa
Lysates were prepared from untreated cells and cells treated with 2µM Doxorubicin used as a DNA damaging agent. The arrow indicates E2F1.
Phosphorylated E2F1 was only expressed in the treated cells. The blot clearly shows that ab5391 only detected phosphorylated E2F1 in the human cell lines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 (phospho S364) antibody (ab5391)
ab5391 at 10 μg/ml staining human breast carcinoma.
E2F1 (phospho S364) antibody for WB in Human (5391)
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