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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F1 (phospho S364) antibody
See all E2F1 products (20) ...
Description
Rabbit polyclonal to E2F1 (phospho S364)
Specificity
This antibody is specific for the phosphorylated form of E2F1. Minimal cross-reactivity is seen with non-phosphorylated human E2F1 by peptide ELISA.
Tested applications
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide: SRMGSpLRAPV-C conjugated to KLH, corresponding to amino acids 360-369 of Human E2F 1.
SRMGSpLRAP V-C
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Target
Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similarities
Belongs to the E2F/DP family.
Post-translational
modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ01094
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 1 antibody
- E2F transcription factor 1 antibody
- E2F-1 antibody
see all
Database links
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
Anti-E2F1 (phospho S364) antibody images:
Western blot - E2F1 (phospho S364) antibody (ab5391)
All lanes : Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution
Lane 1 : CRE (mouse) cells transfected with human E2F1
Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin
Lane 3 : NiH3T3 (mouse) cell lysate
Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin
Lane 5 : MDA-231 (human breast cancer) cell lysate
Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin
Secondary
HRP conjugated Goat-anti-Rabbit IgG MX10
Predicted band size : 47 kDa
Lysates were prepared from untreated cells and cells treated with 2µM Doxorubicin used as a DNA damaging agent. The arrow indicates E2F1.
Phosphorylated E2F1 was only expressed in the treated cells. The blot clearly shows that ab5391 only detected phosphorylated E2F1 in the human cell lines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 (phospho S364) antibody (ab5391)
ab5391 at 10 μg/ml staining human breast carcinoma.
References for Anti-E2F1 (phospho S364) antibody (ab5391)
This product has been referenced in:
- Solier Set al. Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways. Mol Cell Biol 29:68-82 (2009). WB; Human.Read more (PubMed: 18955500) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"