|
|
|
|
Western blot - E2F1 (phospho S364) antibody (ab5391)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 (phospho S364) antibody (ab5391)
(enlarge)
|
|
|
|
|
Product Name
|
E2F1 (phospho S364) antibody
|
| |
See all E2F1 antibodies (17)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to E2F1 (phospho S364)
|
|
Immunogen
|
Synthetic peptide: SRMGSpLRAPV-C conjugated to KLH, corresponding to amino acids 360-369 of Human E2F 1.
BLAST 'SRMGSpLRAPV-C' with  or 
|
|
Reacts with
|
Hu
|
|
Does not react with
(species key)
|
Ms
|
|
Specificity
|
This antibody is specific for the phosphorylated form of E2F1. Minimal cross-reactivity is seen with non-phosphorylated human E2F1 by peptide ELISA.
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/250. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Transcription >> Other factors
|
|
Relevance
|
E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 0.80 - 0.80 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F1 (phospho S364) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for E2F1 (phospho S364) antibody images, references, products related to ab5391 and other tools.
|
| |
E2F1 (phospho S364) antibody images:
|
 Western blot - E2F1 (phospho S364) antibody (ab5391)
All lanes : E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution
Lane 1 : CRE (mouse) cells transfected with human E2F1
Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin
Lane 3 : NiH3T3 (mouse) cell lysate
Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin
Lane 5 : MDA-231 (human breast cancer) cell lysate
Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin
Secondary
HRP conjugated Goat-anti-Rabbit IgG MX10
Predicted band size : 47 kDa
Lysates were prepared from untreated cells and cells treated with 2µM Doxorubicin used as a DNA damaging agent. The arrow indicates E2F1. Phosphorylated E2F1 was only expressed in the treated cells. The blot clearly shows that ab5391 only detected phosphorylated E2F1 in the human cell lines.
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F1 (phospho S364) antibody (ab5391)
ab5391 at 10 μg/ml staining human breast carcinoma.
|
| |
References for E2F1 (phospho S364) antibody (ab5391)
|
|
This product has been used in:
Solier S et al. Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways. Mol Cell Biol 29:68-82 (2009). WB; Human. PubMed: 18955500
If you publish research using ab5391 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to E2F1
|
| |
E2F1 (phospho S364) antibody - more information
|
Customer reviews (feedback) regarding E2F1 (phospho S364) antibody |
Customer FAQs regarding E2F1 (phospho S364) antibody |
Protocols for E2F1 (phospho S364) antibody |
Price and availability of products related to E2F1 (phospho S364) antibody |
| |
E2F1 (phospho S364) antibody - related products:
|
|
|
| |
E2F1 (phospho S364) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about E2F1 (phospho S364) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
