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Western blot - E2F1 (phospho T433) antibody (ab55325)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-E2F1 (phospho T433) antibody(ab55325)
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Product Name
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E2F1 (phospho T433) antibody
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See all E2F1 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to E2F1 (phospho T433)
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Immunogen
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Synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of threonine 433 (D-L-TP-P-L).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody detects endogenous levels of E2F1 only when phosphorylated at threonine 433.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
IHC-P: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell extract.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F1 (phospho T433) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F1 (phospho T433) antibody images, references, products related to ab55325 and other tools.
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E2F1 (phospho T433) antibody images:
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 Western blot - E2F1 (phospho T433) antibody (ab55325)
All lanes : E2F1 (phospho T433) antibody (ab55325) at 1/500 dilution
Lane 1 : HeLa cell extract treated with Etoposide (at 25µM for 24 hrs).
Lane 2 : HeLa cell extract treated with Etoposide (at 25µM for 24 hrs), and with the immunising phosphopeptide.
Predicted band size : 47 kDa
Observed band size : 47 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-E2F1 (phospho T433) antibody(ab55325)
Ab55325 staining human normal pancreas. Staining is localised to nuclear compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to E2F1
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E2F1 (phospho T433) antibody - more information
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