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Anti-E2F2 antibody [CC11] (ab11838)

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Overview

Product name

Anti-E2F2 antibody [CC11]
See all E2F2 products (6) ...

Description

Mouse monoclonal [CC11] to E2F2

Specificity

Does not detect the other members of the E2F family.

Tested applications

IP, WB, Gel supershift assaysmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant protein (Human).

General notes

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

CC11

Isotype

IgG2a

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 53 kDa (predicted molecular weight: 48 kDa).(76 (alpha), 50 (beta, singlet or doublet), 45 (epsilon), 42 (delta), 38 (gamma, singlet or doublet) and 25 kDa (zeta).)

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    Gel supershift assays

     GSA: Use at an assay dep...Read more →

    GSA: Use at an assay dependent dilution.

Target

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.

Tissue specificity

Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

Sequence similarities

Belongs to the E2F/DP family.

Post-translational
modifications

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ14209 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • dE2F2 antibody
  • E2F transcription factor 2 antibody
  • E2F-2 antibody
  • E2F2 antibody
  • E2F2_HUMAN antibody
  • Transcription factor E2F2 antibody
see all

Database links

References for Anti-E2F2 antibody [CC11] (ab11838)

ab11838 has not yet been referenced specifically in any publications.

Publishing research using ab11838? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"