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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - E2F2 antibody (ab14292)
Predicted band size : 52 kDa
E coli-derived fusion protein as test antigen. Affi-anti- E2F2 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. Arrow points to E2F2 fusion protein.
Western blot - E2F2 antibody (ab14292)
All lanes : Anti-E2F2 antibody (ab14292) at 1/3000 dilution
Lane 1 : Rat cardiomyocytes whole cell lysate.
Lane 2 : Rat cardiomyocytes whole cell lysate. Treated with an adenovirus
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6877) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
The primary antibody was diluted in 5% BSA and incubated for 12 hours at 4°C.
This image is courtesy of an anonymous Abreview
E2F2 antibody for WB in Rat (14292)
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