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Western blot - E2F2 antibody (ab14292)
(enlarge)
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Western blot - E2F2 antibody (ab14292)
(enlarge)
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Product Name
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E2F2 antibody
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See all E2F2 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to E2F2
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Immunogen
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Synthetic peptide: corresponding to C terminal amino acids 348-437 of Human E2F2.
BLAST 'svpapaptpq qappppslvp leatdsllel phpllqqted qflsptlacs splisfspsl dqd dylwgl eagegisdlf dsydlgdlli ' with  or 
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Reacts with
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F2 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F2 antibody images, references, products related to ab14292 and other tools.
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E2F2 antibody images:
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 Western blot - E2F2 antibody (ab14292)
Predicted band size : 52 kDa
E coli-derived fusion protein as test antigen. Affi-anti- E2F2 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. Arrow points to E2F2 fusion protein.
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Western blot - E2F2 antibody (ab14292)
All lanes : E2F2 antibody (ab14292) at 1/3000 dilution
Lane 1 : Rat cardiomyocytes whole cell lysate.
Lane 2 : Rat cardiomyocytes whole cell lysate. Treated with an adenovirus
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Chicken IgY - H&L (HRP) (ab6877) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
The primary antibody was diluted in 5% BSA and incubated for 12 hours at 4°C. This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to E2F2
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E2F2 antibody - more information
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Customer reviews (feedback) regarding E2F2 antibody |
Customer FAQs regarding E2F2 antibody |
Protocols for E2F2 antibody |
Price and availability of products related to E2F2 antibody |
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E2F2 antibody - related products:
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E2F2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about E2F2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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