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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F2 antibody
See all E2F2 products (6) ...
Description
Chicken polyclonal to E2F2
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: corresponding to C terminal amino acids 348-437 of Human E2F2.
svpapaptpq qappppslvp leatdsllel phpllqqted qflsptlacs splisfspsl dqd dylwgleage gisdlf dsydlgdlli n
General notes
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/2000.Predicted molecular weight: 52 kDa.
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.
Tissue specificity
Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
Sequence similarities
Belongs to the E2F/DP family.
Post-translational
modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ14209
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dE2F2 antibody
- E2F transcription factor 2 antibody
- E2F transcription factor 2 antibody
see all
Database links
- Entrez Gene: 1870 Human
- Entrez Gene: 242705 Mouse
- Omim: 600426 Human
- SwissProt: Q14209 Human
- SwissProt: P56931 Mouse
- Unigene: 194333 Human
- Unigene: 307932 Mouse
Anti-E2F2 antibody images:
Western blot - E2F2 antibody (ab14292)
Predicted band size : 52 kDa
E coli-derived fusion protein as test antigen. Affi-anti- E2F2 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. Arrow points to E2F2 fusion protein.
Western blot - E2F2 antibody (ab14292)
All lanes : Anti-E2F2 antibody (ab14292) at 1/3000 dilution
Lane 1 : Rat cardiomyocytes whole cell lysate.
Lane 2 : Rat cardiomyocytes whole cell lysate. Treated with an adenovirus
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6877) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
The primary antibody was diluted in 5% BSA and incubated for 12 hours at 4°C.
This image is courtesy of an anonymous Abreview
References for Anti-E2F2 antibody (ab14292)
ab14292 has not yet been referenced specifically in any publications.
Publishing research using ab14292? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"