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Anti-E2F2 antibody (ab36752)

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Overview

Product name

Anti-E2F2 antibody
See all E2F2 products (6) ...

Description

Rabbit polyclonal to E2F2

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to N-terminal of human E2F2.

Positive control

NIH3T3 cells. Breast carcinoma.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box

  • Western blot - E2F2 antibody (ab36752)Western blot - E2F2 antibody (ab36752) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1 - 2 µg/ml for 2 hours at RT. Predicted molecular weight: 55 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.

Tissue specificity

Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

Sequence similarities

Belongs to the E2F/DP family.

Post-translational
modifications

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ14209 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • dE2F2 antibody
  • E2F transcription factor 2 antibody
  • E2F-2 antibody
  • E2F2 antibody
  • E2F2_HUMAN antibody
  • Transcription factor E2F2 antibody
see all

Database links

Anti-E2F2 antibody images:

  Western blot - E2F2 antibody (ab36752)

Western blot - E2F2 antibody (ab36752)

Anti-E2F2 antibody (ab36752) at 1/25 dilution + lysate prepared from HepG2 cells

Predicted band size : 55 kDa
Observed band size : 55 kDa

References for Anti-E2F2 antibody (ab36752)

ab36752 has not yet been referenced specifically in any publications.

Publishing research using ab36752? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"