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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F2 antibody
See all E2F2 products (6) ...
Description
Rabbit polyclonal to E2F2
Specificity
ab65222 detects endogenous levels of total E2F2 protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from an internal sequence of human E2F2.
Positive control
Extracts from HepG2 cells, K562 cells and COLO205 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab65222 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - E2F2 antibody (ab65222)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
WB: 1/500 - 1/1000.Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.
Tissue specificity
Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
Sequence similarities
Belongs to the E2F/DP family.
Post-translational
modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ14209
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dE2F2 antibody
- E2F transcription factor 2 antibody
- E2F-2 antibody
see all
Database links
- Entrez Gene: 1870 Human
- Entrez Gene: 242705 Mouse
- Omim: 600426 Human
- SwissProt: Q14209 Human
- SwissProt: P56931 Mouse
- Unigene: 194333 Human
- Unigene: 307932 Mouse
Anti-E2F2 antibody images:
Western blot - E2F2 antibody (ab65222)
All lanes : Anti-E2F2 antibody (ab65222) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : K562 cell extract
Lane 3 : COLO205 cell extract
Lane 4 : COLO205 cell extract with immunising peptide
at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 48 kDa
Observed band size : 48 kDa
References for Anti-E2F2 antibody (ab65222)
This product has been referenced in:
- Wan Zet al. Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors. J Clin Invest 120:3530-44 (2010). WB; Human.Read more (PubMed: 20890043) »
See 1 publication for this product
Publishing research using ab65222? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"