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Western blot - E2F2 antibody (ab65222)
(enlarge)
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Product Name
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E2F2 antibody
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See all E2F2 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to E2F2
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Immunogen
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Synthetic peptide derived from an internal sequence of human E2F2.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab65222 detects endogenous levels of total E2F2 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HepG2 cells, K562 cells and COLO205 cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F2 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab65222 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F2 antibody images, references, products related to ab65222 and other tools.
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E2F2 antibody images:
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 Western blot - E2F2 antibody (ab65222)
All lanes : E2F2 antibody (ab65222) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : K562 cell extract
Lane 3 : COLO205 cell extract
Lane 4 : COLO205 cell extract with immunising peptide
at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 48 kDa
Observed band size : 48 kDa
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Search PubMed (MEDLINE) for references to E2F2
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E2F2 antibody - more information
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Customer reviews (feedback) regarding E2F2 antibody |
Customer FAQs regarding E2F2 antibody |
Protocols for E2F2 antibody |
Price and availability of products related to E2F2 antibody |
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E2F2 antibody - related products:
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E2F2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about E2F2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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