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Western blot - E2F3 antibody (ab50917)
(enlarge)
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Product Name
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E2F3 antibody
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See all E2F3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to E2F3
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Immunogen
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Synthetic peptide corresponding to a region of Human E2F3
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/62500
WB: Use at a concentration of 0.25 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal thymus lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F3 antibody images, references, products related to ab50917 and other tools.
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E2F3 antibody images:
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 Western blot - E2F3 antibody (ab50917)
E2F3 antibody (ab50917) at 0.25 µg/ml + Fetal thymus lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 49 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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References for E2F3 antibody (ab50917)
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This product has been used in:
Naga Prasad SV et al. Unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks. J Biol Chem 284:27487-99 (2009). WB; Human. PubMed: 19641226
If you publish research using ab50917 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to E2F3
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E2F3 antibody - more information
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Customer reviews (feedback) regarding E2F3 antibody |
Customer FAQs regarding E2F3 antibody |
Protocols for E2F3 antibody |
Price and availability of products related to E2F3 antibody |
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E2F3 antibody - related products:
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E2F3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about E2F3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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