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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F3 antibody
See all E2F3 products (4) ...
Description
Mouse monoclonal to E2F3
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: QIHLASTQGP IEVYLCPEET ETHSPMKTNN QDHNGNIPKP ASKDLASTNS GHSDCSVSMG NLSPLASPAN LLQQTEDQIP SNLEGPFVNL , corresponding to amino acids 336-426 of Human E2F3
QIHLASTQGP IEVYLCPEET ETHSPMKTNN QDHNGNIPKP ASKDLASTNS GHSDCSVSMG NLSPLASPAN LLQQTEDQIP SNLEGPFVNL
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
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Western blot - E2F3 antibody (ab54945)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.
Sequence similarities
Belongs to the E2F/DP family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00716
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686C18211 antibody
- E2F 3 antibody
- E2F transcription factor 3 antibody
see all
Database links
- Entrez Gene: 1871 Human
- GenBank: AAH16847 Human
- Omim: 600427 Human
- SwissProt: O00716 Human
- SwissProt: Q24JQ3 Human
- SwissProt: Q499G5 Human
- Unigene: 1189 Human
- Unigene: 269408 Human
see all
Anti-E2F3 antibody images:
Western blot - E2F3 antibody (ab54945)
E2F3 antibody (ab54945) at 1ug/lane + COLO cell lysate at 25ug/lane.
References for Anti-E2F3 antibody (ab54945)
This product has been referenced in:
- De Guire Vet al. Designing small multiple-target artificial RNAs. Nucleic Acids Res 38:e140 (2010). WB; Human.Read more (PubMed: 20453028) »
See 1 publication for this product
Publishing research using ab54945? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"