![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [4E2F04] (ab4071) image](#ab4071_1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F4 antibody [4E2F04]
See all E2F4 products (7) ...
Description
Mouse monoclonal [4E2F04] to E2F4
Specificity
Protein of 56 kDa.
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Recombinant full length protein (Human).
Positive control
Tonsil.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Ascites
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
4E2F04
Isotype
IgG1
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [4E2F04] (ab4071)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [4E2F04] (ab4071) image](/ps/datasheet/Images/4/ab4071/ab4071_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
IHC-Fr
Application notes
Immunohistochemistry: 1/25 - 1/50 for 30mins at RT (ABC method).
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificity
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the E2F/DP family.
Developmental stage
Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
Post-translational
modifications
Differentially phosphorylated in vivo.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ16254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
see all
Database links
- Entrez Gene: 1874 Human
- Entrez Gene: 307794 Rat
- Omim: 600659 Human
- SwissProt: Q16254 Human
- Unigene: 108371 Human
Anti-E2F4 antibody [4E2F04] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [4E2F04] (ab4071)
ab4071 staining human tonsil by IHC-P.
References for Anti-E2F4 antibody [4E2F04] (ab4071)
ab4071 has not yet been referenced specifically in any publications.
Publishing research using ab4071? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"