![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [SPM179] (ab54168) image](#ab54168ihc.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F4 antibody [SPM179], prediluted
See all E2F4 products (7) ...
Description
Mouse monoclonal [SPM179] to E2F4, prediluted
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rat
Immunogen
Recombinant Human E2F4
Positive control
Raji cells, Tonsil.
Properties
Form
Prediluted
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
SPM179
Isotype
IgG1
Light chain type
kappa
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [SPM179] (ab54168)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [SPM179] (ab54168) image](/ps/datasheet/Images/54/ab54168/ab54168ihc.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab54168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: ready to use.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificity
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the E2F/DP family.
Developmental stage
Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
Post-translational
modifications
Differentially phosphorylated in vivo.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ16254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
see all
Database links
- Entrez Gene: 1874 Human
- Entrez Gene: 307794 Rat
- Omim: 600659 Human
- SwissProt: Q16254 Human
- Unigene: 108371 Human
Anti-E2F4 antibody [SPM179], prediluted images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E2F4 antibody [SPM179] (ab54168)
immunohistochemical staining of formalin fixed, paraffin embedded human tonsil tissue section using ab54168.
References for Anti-E2F4 antibody [SPM179], prediluted (ab54168)
ab54168 has not yet been referenced specifically in any publications.
Publishing research using ab54168? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"