![Western blot - E2F4 antibody [WUF11] (ab11951) image](#11951.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F4 antibody [WUF11]
See all E2F4 products (7) ...
Description
Mouse monoclonal [WUF11] to E2F4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full-length protein corresponding to human E2F-4.
Positive control
K-562 nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.2% Gelatin, 0.01% Thimerosal and 0.1% Sodium Azide
Constituents: PBS
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
WUF11
Isotype
IgG1
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
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Western blot - E2F4 antibody [WUF11] (ab11951)
![Western blot - E2F4 antibody [WUF11] (ab11951) image](/ps/datasheet/Images/11/ab11951/11951.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab11951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Uncharacterized bands of lower and higher molecular weight may also be revealed under similar conditions. For optimal results primary antibody incubations should be performed at 37°C
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificity
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the E2F/DP family.
Developmental stage
Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
Post-translational
modifications
Differentially phosphorylated in vivo.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ16254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
see all
Database links
- Entrez Gene: 1874 Human
- Omim: 600659 Human
- SwissProt: Q16254 Human
- Unigene: 108371 Human
Anti-E2F4 antibody [WUF11] images:
Western blot - E2F4 antibody [WUF11] (ab11951)
Predicted band size : 45 kDa
The presence of E2F-4 in K-562 nuclear extracts is confirmed by the detection of a signal corresponding to a protein of approximately 60 kD using ab11951 at a 1/1000 dilution.
References for Anti-E2F4 antibody [WUF11] (ab11951)
ab11951 has not yet been referenced specifically in any publications.
Publishing research using ab11951? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"