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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F4 antibody [WUF3]
See all E2F4 products (7) ...
Description
Mouse monoclonal [WUF3] to E2F4
Tested applications
WB, IP, Gel supershift assaysmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant protein (Human).
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
WUF3
Isotype
IgG1
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
Gel supershift assays
Application notes
GSA: Use at an assay dependent dilution.
IP (denatured): Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1 µ g/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F4. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa) (for natural molecule, may vary in a recombinant preparation).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificity
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the E2F/DP family.
Developmental stage
Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
Post-translational
modifications
Differentially phosphorylated in vivo.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ16254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
see all
Database links
- Entrez Gene: 1874 Human
- Omim: 600659 Human
- SwissProt: Q16254 Human
- Unigene: 108371 Human
References for Anti-E2F4 antibody [WUF3] (ab11845)
ab11845 has not yet been referenced specifically in any publications.
Publishing research using ab11845? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"