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Western blot - E2F4 antibody (ab53060)
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Immunohistochemistry (Paraffin-embedded sections) - E2F4 antibody (ab53060)
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Product Name
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E2F4 antibody
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See all E2F4 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to E2F4
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Immunogen
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A synthesized peptide derived from human E2F4.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody detects endogenous levels of total E2F4 protein.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IHC-P: Human lung carcinoma tissue
WB: Extracts from COS7 cells.
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2Fs are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinitypurified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F4 antibody images, references, products related to ab53060 and other tools.
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E2F4 antibody images:
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 Western blot - E2F4 antibody (ab53060)
All lanes : E2F4 antibody (ab53060) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells treated with peptide
Predicted band size : 44 kDa
Observed band size : 44 kDa
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Immunohistochemistry (Paraffin-embedded sections) - E2F4 antibody (ab53060)
Immunohistochemical analysis of E2F4 in paraffin embedded
human lung carcinoma tissue using ab53060 at a 1/50 dilution.
Left image untreated.
Right image treated with peptide.
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Search PubMed (MEDLINE) for references to E2F4
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E2F4 antibody - more information
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Customer reviews (feedback) regarding E2F4 antibody |
Customer FAQs regarding E2F4 antibody |
Protocols for E2F4 antibody |
Price and availability of products related to E2F4 antibody |
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E2F4 antibody - related products:
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E2F4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about E2F4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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