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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F4 antibody
See all E2F4 products (7) ...
Description
Rabbit polyclonal to E2F4
Specificity
This antibody detects endogenous levels of total E2F4 protein.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthesized peptide derived from human E2F4.
Positive control
IHC-P: Human lung carcinoma tissue WB: Extracts from COS7 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinitypurified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
Target
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificity
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the E2F/DP family.
Developmental stage
Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
Post-translational
modifications
Differentially phosphorylated in vivo.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ16254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
see all
Database links
- Entrez Gene: 1874 Human
- Entrez Gene: 104394 Mouse
- Entrez Gene: 307794 Rat
- Omim: 600659 Human
- SwissProt: Q16254 Human
- SwissProt: Q8R0K9 Mouse
- Unigene: 108371 Human
- Unigene: 34554 Mouse
Anti-E2F4 antibody images:
Western blot - E2F4 antibody (ab53060)
All lanes : Anti-E2F4 antibody (ab53060) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells treated with peptide
Predicted band size : 44 kDa
Observed band size : 44 kDa
Immunohistochemistry (Paraffin-embedded sections) - E2F4 antibody (ab53060)
Immunohistochemical analysis of E2F4 in paraffin embedded
human lung carcinoma tissue using ab53060 at a 1/50 dilution.
Left image untreated.
Right image treated with peptide.
References for Anti-E2F4 antibody (ab53060)
ab53060 has not yet been referenced specifically in any publications.
Publishing research using ab53060? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"