E2F5 antibody [220D1a] (ab59769)

Product Name 

E2F5 antibody [220D1a]

Product type 

Primary antibodies

Description 

Mouse monoclonal [220D1a] to E2F5

Immunogen 

Recombinant fragment (Human)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

Dot, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.
The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.
E2F5 is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. E2F5 is probably involved in the early responses of resting cells to growth factor stimulation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

220D1a

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Filtered through a 0.22 µm membrane.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for E2F5 antibody [220D1a] images, references, products related to ab59769 and other tools.

E2F5 antibody [220D1a] images:

Customer reviews (feedback) regarding E2F5 antibody [220D1a]

Customer FAQs regarding E2F5 antibody [220D1a]

Protocols for E2F5 antibody [220D1a]

Price and availability of products related to E2F5 antibody [220D1a]

E2F5 antibody [220D1a] - related products: