Feedback Form

Western blot - E2F5 antibody [220D1a] (ab59769)
Western blot - E2F5 antibody [220D1a] (ab59769)
(enlarge)


Product Name 

E2F5 antibody [220D1a]

 

See all E2F5 antibodies (3)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [220D1a] to E2F5

Immunogen 

Recombinant fragment (Human)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

Dot, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.
The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.
E2F5 is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. E2F5 is probably involved in the early responses of resting cells to growth factor stimulation.

 

Alternative names E2F5 antibody [220D1a] (ab59769)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1875    (Human)

Omim

    

600967    (Human)

SwissProt

    

Q15329    (Human)

Unigene

    

445758    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

220D1a

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Filtered through a 0.22 µm membrane.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F5 antibody [220D1a] is on this datasheet. But please do contact us if you would like any reassurance!


See below for E2F5 antibody [220D1a] images, references, products related to ab59769 and other tools.

 

E2F5 antibody [220D1a] images:

Product image  Western blot - E2F5 antibody [220D1a] (ab59769)

Western blot - E2F5 antibody [220D1a] (ab59769)

E2F5 antibody [220D1a] (ab59769) + immunizing peptide

Predicted band size : 38 kDa
Observed band size : 38 kDa

 
 

Search PubMed (MEDLINE) for references to E2F5

 

E2F5 antibody [220D1a] - more information

Customer reviews (feedback) regarding E2F5 antibody [220D1a]

Customer FAQs regarding E2F5 antibody [220D1a]

Protocols for E2F5 antibody [220D1a]

Price and availability of products related to E2F5 antibody [220D1a]

 

E2F5 antibody [220D1a] - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for E2F5 antibody [220D1a]

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other E2F5 Antibodies

See all E2F5 antibodies (3)...

 

E2F5 antibody [220D1a] - other tools:

Contact Abcam with a Technical Enquiry about E2F5 antibody [220D1a]

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.