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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-E2F5 antibody
See all E2F5 products (3) ...
Description
Rabbit polyclonal to E2F5
Specificity
ab22855 recognises Transcription factor E2F5.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken
Immunogen
Synthetic peptide derived from a region between residues 126-180 of Human E2F5.
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
-
Western blot - E2F 5 antibody (ab22855)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.4 µg/mlDetects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
Target
Function
Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation.
Sequence similarities
Belongs to the E2F/DP family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ15329
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 5 antibody
- E2F transcription factor 5 antibody
- E2F transcription factor 5 p130 binding antibody
see all
Database links
- Entrez Gene: 420206 Chicken
- Entrez Gene: 1875 Human
- Entrez Gene: 13559 Mouse
- Entrez Gene: 116651 Rat
- Omim: 600967 Human
- SwissProt: Q15329 Human
- SwissProt: Q61502 Mouse
- SwissProt: Q99LK0 Mouse
see all
Anti-E2F5 antibody images:
Western blot - E2F 5 antibody (ab22855)
Anti-E2F5 antibody (ab22855) at 0.4 µg/ml + Jurka cell lysate with 5% skim milk in PBS
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 38 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-E2F5 antibody (ab22855)
ab22855 has not yet been referenced specifically in any publications.
Publishing research using ab22855? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"