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Western blot - E2F 5 antibody (ab22855)
(enlarge)
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Product Name
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E2F5 antibody
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See all E2F5 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to E2F5
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Immunogen
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Synthetic peptide derived from a region between residues 126-180 of Human E2F5.
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Reacts with
(species key)
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Hu
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Specificity
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ab22855 recognises Transcription factor E2F5.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.4 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.
The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.
E2F5 is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. E2F5 is probably involved in the early responses of resting cells to growth factor stimulation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F5 antibody images, references, products related to ab22855 and other tools.
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E2F5 antibody images:
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 Western blot - E2F 5 antibody (ab22855)
E2F5 antibody (ab22855) at 0.4 µg/ml + Jurka cell lysate with 5% skim milk in PBS
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 38 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to E2F5
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E2F5 antibody - more information
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Customer reviews (feedback) regarding E2F5 antibody |
Customer FAQs regarding E2F5 antibody |
Protocols for E2F5 antibody |
Price and availability of products related to E2F5 antibody |
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E2F5 antibody - related products:
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E2F5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about E2F5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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