![Western blot - E2F6 antibody [TFE61] (ab11952) image](#ACFAF8.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F6 antibody [TFE61]
See all E2F6 products (4) ...
Description
Mouse monoclonal [TFE61] to E2F6
Tested applications
IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human)
Positive control
Hela cells or K-562 nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
TFE61
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
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Western blot - E2F6 antibody [TFE61] (ab11952)
![Western blot - E2F6 antibody [TFE61] (ab11952) image](/ps/datasheet/Images/11/ab11952/ACFAF8.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab11952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
ICC/IF
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 38 kDa from K-562 nuclear extract(predicted molecular weight: 32 kDa). For optimal results, primary antibody incubations should be performed at 37°C
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Tissue specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Sequence similarities
Belongs to the E2F/DP family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75461
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Database links
- Entrez Gene: 1876 Human
- Omim: 602944 Human
- SwissProt: O75461 Human
- Unigene: 603093 Human
Anti-E2F6 antibody [TFE61] images:
Western blot - E2F6 antibody [TFE61] (ab11952)
Predicted band size : 32 kDa
Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.
References for Anti-E2F6 antibody [TFE61] (ab11952)
This product has been referenced in:
- Furuta Met al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB; Human.Read more (PubMed: 19843643) »
- Kozaki Ket al. Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res 68:2094-105 (2008). WB; Human.Read more (PubMed: 18381414) »
See all 2 publications for this product
Publishing research using ab11952? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"