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Western blot - E2F6 antibody [TFE61] (ab11952)
Western blot - E2F6 antibody [TFE61] (ab11952)
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Product Name 

E2F6 antibody [TFE61]

 

See all E2F6 antibodies (3)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [TFE61] to E2F6

Immunogen 

Recombinant full length protein (Human)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 38 kDa from K-562 nuclear extract(predicted molecular weight: 32 kDa). For optimal results, primary antibody incubations should be performed at 37°C

ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Hela cells or K-562 nuclear extract.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

The E2F6 gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses.

 

Alternative names E2F6 antibody [TFE61] (ab11952)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1876    (Human)

GeneCard

    

GC02M011636    (Human)

Omim

    

602944    (Human)

SwissProt

    

O75461    (Human)

Unigene

    

603093    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

TFE61

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F6 antibody [TFE61] is on this datasheet. But please do contact us if you would like any reassurance!


See below for E2F6 antibody [TFE61] images, references, products related to ab11952 and other tools.

 

E2F6 antibody [TFE61] images:

Product image  Western blot - E2F6 antibody [TFE61] (ab11952)

Western blot - E2F6 antibody [TFE61] (ab11952)



Predicted band size : 32 kDa


Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.

 

References for E2F6 antibody [TFE61] (ab11952)

Specific publication references   This product has been used in:

Kozaki K et al. Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res 68:2094-105 (2008). WB; Human. PubMed: 18381414

If you publish research using ab11952 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to E2F6

 

E2F6 antibody [TFE61] - more information

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