Overview

  • Product nameAnti-E2F6 antibody [TFE61]
    See all E2F6 primary antibodies
  • Description
    Mouse monoclonal [TFE61] to E2F6
  • Tested applicationsIP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Hela cells or K-562 nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab11952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
ICC/IF
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 38 kDa from K-562 nuclear extract(predicted molecular weight: 32 kDa). For optimal results, primary antibody incubations should be performed at 37°C

    ICC/IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
    • Tissue specificityExpressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
    • Sequence similaritiesBelongs to the E2F/DP family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2F 6 antibody
      • E2F binding site modulating activity protein antibody
      • E2F transcription factor 6 antibody
      • E2F transcription factor 6 isoform 1 antibody
      • E2F-6 antibody
      • E2F6 antibody
      • E2F6_HUMAN antibody
      • EMA antibody
      • MGC111545 antibody
      • Transcription factor E2F6 antibody
      see all

    Anti-E2F6 antibody [TFE61] images



    • Predicted band size : 32 kDa


      Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.

    References for Anti-E2F6 antibody [TFE61] (ab11952)

    This product has been referenced in:
    • Furuta M  et al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB ; Human . Read more (PubMed: 19843643) »
    • Kozaki K  et al. Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res 68:2094-105 (2008). WB ; Human . Read more (PubMed: 18381414) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab11952.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"