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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F6 antibody
See all E2F6 products (4) ...
Description
Rabbit polyclonal to E2F6
Specificity
This antibody detects endogenous levels of total E2F6 protein.
Tested applications
IP, WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A synthesized peptide derived from human E2F6.
Positive control
Extracts from HepG2 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 20439742)
WB: 1/500 - 1/1000.Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Tissue specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Sequence similarities
Belongs to the E2F/DP family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75461
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Database links
- Entrez Gene: 1876 Human
- Entrez Gene: 50496 Mouse
- Omim: 602944 Human
- SwissProt: O75461 Human
- SwissProt: O54917 Mouse
- Unigene: 603093 Human
- Unigene: 23296 Mouse
Anti-E2F6 antibody images:
Western blot - E2F6 antibody (ab53061)
All lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution
Lane 1 : Extracts of HepG2 cells
Lane 2 : Extracts of HepG2 cells treated with peptide
Predicted band size : 32 kDa
Observed band size : 32 kDa
Western blot - Anti-E2F6 antibody (ab53061)
All lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution (in TBS fro 16 hours at 4°C )
Lane 1 : HeLa cells
Lane 2 : Cell lysate (nuclear) of mouse embryonic stem cells at 100000 cells
Secondary
HRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 32 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
Blocking Step: 4% Milk for 1 hour at 22°C
This image is courtesy of an Abreview submitted by Dr. Ligia Tavares
References for Anti-E2F6 antibody (ab53061)
This product has been referenced in:
- Velasco Get al. Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. Proc Natl Acad Sci U S A 107:9281-6 (2010). WB, IP; Human.Read more (PubMed: 20439742) »
See 1 publication for this product
Publishing research using ab53061? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"