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Western blot - E2F6 antibody (ab53061)
Western blot - E2F6 antibody (ab53061)
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Product Name 

E2F6 antibody

 

See all E2F6 antibodies (3)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to E2F6

Immunogen 

A synthesized peptide derived from human E2F6.

Reacts with 
(species key)

Hu, Ms

Specificity 

This antibody detects endogenous levels of total E2F6 protein.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from HepG2 cells.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

The E2F6 gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses.

 

Alternative names E2F6 antibody (ab53061)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

1876    (Human)
50496    (Mouse)

Omim

    

602944    (Human)

SwissProt

    

O75461    (Human)
O54917    (Mouse)

Unigene

    

603093    (Human)
23296    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F6 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for E2F6 antibody images, references, products related to ab53061 and other tools.

 

E2F6 antibody images:

Product image  Western blot - E2F6 antibody (ab53061)

Western blot - E2F6 antibody (ab53061)

All lanes : E2F6 antibody (ab53061) at 1/500 dilution

Lane 1 : Extracts of HepG2 cells
Lane 2 : Extracts of HepG2 cells treated with peptide


Predicted band size : 32 kDa
Observed band size : 32 kDa

 
 

Search PubMed (MEDLINE) for references to E2F6

 

E2F6 antibody - more information

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E2F6 antibody - related products:

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Loading controls

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ab14660

  

HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

 

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