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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F6 antibody
See all E2F6 products (4) ...
Description
Rabbit polyclonal to E2F6
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide: KMVMIQDGPQ NTGADKPLRI TGDPYKVQQA KEMVLELIRD QGGFREVRNE , corresponding to amino acids 218-267 of Human E2F6
KMVMIQDGPQ NTGADKPLRI TGDPYKVQQA KEMVLELIRD QGGFREVRNE
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
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Western blot - E2F6 antibody (ab66609)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/1562500.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of <36 kDa (predicted molecular weight: 32 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Tissue specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Sequence similarities
Belongs to the E2F/DP family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75461
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Database links
- Entrez Gene: 514085 Cow
- Entrez Gene: 609996 Dog
- Entrez Gene: 1876 Human
- Entrez Gene: 50496 Mouse
- Entrez Gene: 313978 Rat
- Omim: 602944 Human
- SwissProt: Q08DY6 Cow
- SwissProt: O75461 Human
see all
Anti-E2F6 antibody images:
Western blot - E2F6 antibody (ab66609)
Anti-E2F6 antibody (ab66609) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 32 kDa
Observed band size : <36 kDa (why is the actual band size different from the predicted?)
Gel concentration: 15%
References for Anti-E2F6 antibody (ab66609)
ab66609 has not yet been referenced specifically in any publications.
Publishing research using ab66609? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"