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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-E2F7 antibody
See all E2F7 products (3) ...
Description
Rabbit polyclonal to E2F7
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog
Immunogen
Synthetic peptide (Mouse)
Positive control
SP2/0 cell lysate and mouse stomach tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
IHC-P
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Along with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity). Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
Sequence similarities
Belongs to the E2F/DP family.
Domain
Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ96AV8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
There are 2 isoforms produced by alternative splicing.
Alternative names
- E2F 7 antibody
- E2F transcription factor 7 antibody
- E2F-7 antibody
see all
Database links
- Entrez Gene: 144455 Human
- Entrez Gene: 52679 Mouse
- Entrez Gene: 314818 Rat
- Omim: 612046 Human
- SwissProt: Q96AV8 Human
- SwissProt: Q6S7F2 Mouse
- Unigene: 416375 Human
- Unigene: 11747 Mouse
see all
Anti-E2F7 antibody images:
Western blot - E2F7 antibody (ab56022)
Anti-E2F7 antibody (ab56022) at 0.5 µg/ml + SP2/0 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 99 kDa
Observed band size : 99 kDa
Immunohistochemistry (Paraffin-embedded sections) - E2F7 antibody (ab56022)
This image shows ab56022 at 4-8µg/ml staining mouse stomach tissue. The arrows point to epithelial cells of the fundic gland (positive label). X400 magnification.
References for Anti-E2F7 antibody (ab56022)
This product has been referenced in:
- Bhattacharyya Set al. Egr-1 induces a profibrotic injury/repair gene program associated with systemic sclerosis. PLoS One 6:e23082 (2011). IHC-P; Human.Read more (PubMed: 21931594) »
See 1 publication for this product
Publishing research using ab56022? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"