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Western blot - E2F7 antibody (ab56022)
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Immunohistochemistry (Paraffin-embedded sections) - E2F7 antibody (ab56022)
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Product Name
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E2F7 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to E2F7
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Immunogen
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Synthetic peptide (Mouse)
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Reacts with
(species key)
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Ms
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SP2/0 cell lysate and mouse stomach tissue.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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E2F7 is an inhibitor of E2F-dependent transcription. It regulates a subset of E2F-dependent genes whose products are required for normal cell cycle progession as well as mitigate cellular proliferation of mouse embryo fibroblasts.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 52679 (Mouse)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this E2F7 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for E2F7 antibody images, references, products related to ab56022 and other tools.
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E2F7 antibody images:
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 Western blot - E2F7 antibody (ab56022)
E2F7 antibody (ab56022) at 0.5 µg/ml + SP2/0 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 99 kDa
Observed band size : 99 kDa
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Immunohistochemistry (Paraffin-embedded sections) - E2F7 antibody (ab56022)
This image shows ab56022 at 4-8µg/ml staining mouse stomach tissue. The arrows point to epithelial cells of the fundic gland (positive label). X400 magnification.
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Search PubMed (MEDLINE) for references to E2F7
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E2F7 antibody - more information
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Customer reviews (feedback) regarding E2F7 antibody |
Customer FAQs regarding E2F7 antibody |
Protocols for E2F7 antibody |
Price and availability of products related to E2F7 antibody |
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E2F7 antibody - related products:
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E2F7 antibody - other tools:
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Contact Abcam with a Technical Enquiry about E2F7 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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