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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-E2F8 antibody
See all E2F8 products (3) ...
Description
Mouse monoclonal to E2F8
Tested applications
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-432 of Human E2F8
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
-
Western blot - E2F8 antibody (ab57775)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
Sequence similarities
Belongs to the E2F/DP family.
Domain
Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
Cellular localization
Nucleus.
Target information above from: UniProt accessionA0AVK6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 8 antibody
- E2F family member 82 antibody
- E2F transcription factor 8 antibody
see all
Database links
- Entrez Gene: 79733 Human
- SwissProt: A0AVK6 Human
- Unigene: 523526 Human
Anti-E2F8 antibody images:
Western blot - E2F8 antibody (ab57775)
Western blot against tagged recombinant protein immunogen using ab57775 E2F8 antibody at 1ug/ml. Predicted band size of immunogen is 74 kDa
References for Anti-E2F8 antibody (ab57775)
This product has been referenced in:
- Wan Zet al. Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors. J Clin Invest 120:3530-44 (2010). WB; Human.Read more (PubMed: 20890043) »
See 1 publication for this product
Publishing research using ab57775? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"