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Overview

  • Product nameE4F1 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 137-588 (452 amino acids) of Human E4F1 with an N terminal His tag. Predicted MWt: 50 kDa, UniProt ID: Q66K89.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 87 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesLVGGGHIKEVIVAAEAELGDGEMAEAPGSPRQQGLGLAGE GEQAQVKLLVNKD GRYVCALCHKTFKTGSILKAHMVTH SSRKDHECKLCGASFRTKGSLIRHHRRH TDERPYKCSK CGKSFRESGALTRHLKSLTPCTEKIRFSVSKDVVVSKEDA RAG SGAGAAGLGTATSSVTGEPIETSPVIHLVTDAKGT VIHEVHVQMQELSLGMKA LAPEPPVSQELPCSSEGSRE NLLHQAMQNSGIVLERAAGEEGALEPAPAAGSS PQPLA VAAPQLPVLEVQPLETQVASEASAVPRTHPCPQCSETFPT AATLEAHK RGHTGPRPFACAQCGKAFPKAYLLKKHQEV HVRERRFRCGDCGKLYKTIAHVR GHRRVHSDERPYPCP KCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKG SLVRHVRHHTGEKPFKCYKCGRGFAEHG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91909 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        E4FE4F transcription factor 1, isoform CRA_bP120E4F transcription factor
  • RelevanceE4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation.
  • Cellular localizationNuclear

    • E4F1 protein (Tagged-His Tag) images

    • SDS-PAGE - E4F1 protein (Tagged-His Tag) (ab91909)
      SDS-PAGE - E4F1 protein (Tagged-His Tag) (ab91909)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for E4F1 protein (Tagged-His Tag) (ab91909)

    ab91909 has not yet been referenced specifically in any publications.

    Publishing research using ab91909 ? Please let us know so that we can cite the reference in this datasheet

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