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Anti-EAAT1 antibody [10D4] - Carboxyterminal end (ab49643)

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Overview

Product name

Anti-EAAT1 antibody [10D4] - Carboxyterminal end
See all EAAT1 products (5) ...

Description

Mouse monoclonal [10D4] to EAAT1 - Carboxyterminal end

Tested applications

IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant protein corresponding to the C terminal region of human EAAT1

Positive control

Immunohistochemistry - Cerebellum

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

10D4

Myeloma

P3-NS1/1-Ag4-1

Isotype

IgG1

Research areas

Neuroscience >> Neurotransmitter >> Transporters >> Glutamate
Neuroscience >> Neurotransmitter >> Amino Acids >> Aspartate
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate

Applications

Show applications key

Our Abpromise guarantee covers the use of ab49643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-Fr

     IHC-Fr: 1/20 - 1/160.

    IHC-Fr: 1/20 - 1/160.

  • ShowHide

    IHC-P

     IHC-P: 1/20 - 1/160.(Hig...Read more →

    IHC-P: 1/20 - 1/160.(High temperature antigen retrieval is recommended.)

Application notes

Is unsuitable for or WB.

Target

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Tissue specificity

Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Involvement in disease

Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.

Post-translational
modifications

Glycosylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionP43003 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EA6 antibody
  • EAA1_HUMAN antibody
  • EAAT1 antibody
  • Excitatory amino acid transporter 1 antibody
  • FLJ25094 antibody
  • GLAST antibody
  • GLAST-1 antibody
  • GLAST1 antibody
  • Glial high affinity glutamate transporter antibody
  • High affinity neuronal glutamate transporter antibody
  • Slc1a3 antibody
  • Sodium dependent glutamate/aspartate transporter antibody
  • Sodium-dependent glutamate/aspartate transporter 1 antibody
  • Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
  • Solute carrier family 1 member 3 antibody
see all

Database links

References for Anti-EAAT1 antibody [10D4] - Carboxyterminal end (ab49643)

This product has been referenced in:

  • Fietz SAet al. OSVZ progenitors of human and ferret neocortex are epithelial-like and expand by integrin signaling. Nat Neurosci 13:690-9 (2010). IHC-P, IHC-Fr; Human, Ferret.Read more (PubMed: 20436478) »

See 1 publication for this product

Publishing research using ab49643? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"