Anti-EAAT1 antibody (ab416)

This antibody is showing non-specific bands in WB.

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement from a different lot.

I apologize, but I misspoke on the phone. This item will be shipping from our UK office, so you can expect to receive it on April 16th once it transfers to our Boston office.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

needs immunogen sequence for reviewers in order to publish paper
alternatively, a sequence range would help too

ANSWER:

Thank you for contacting us.

I received the following information regarding your question:

The immunogen used to develop theantibody ab416is located in the last 100 residues of the rat Excitatory amino acid transporter 1.

We know that the peptide is 20 residues long.

Unfortunately, more detailed information is not available and the actual sequence is considered proprietary.

But I hope that this information is still of some help to you. Please do not hesitate to contact us if you need any more advice or information.

I see that there is a peptide available as ab42682 for anti-EAAT1 ab (ab41751). I would like to purchase a peptide for ab416. Is there one available?

ANSWER:

Thank you for contacting Abcam.

We will be able to offer the peptide for the product ab416. I am presently assembling the information needed to add this to our catalog. It will take a few days for this product to be added to our catalog, however I will let you know the insttant this has been accomplished.

I hope this information is helpful. Please let me know if you have any questions.

Could you send me the protein sequence of product code ab416 - EAAT1, rabbit polyclonal?

ANSWER:

Thank you for contacting us.

I am sorry but the specific immunogen sequence for this product is proprietary. I apologize for any inconvenience this has caused.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Hello,

I would like to use an anti-GLAST antibody in in-cell western assays, to detect the human, rat or mouse GLAST.
Which of your antibodies would you recommend for such an application. ab416 or ab41751?

Thanks for your help.

Best regards,

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ANSWER:

Thank you for your inquiry.
I cannot recommend ab41751 since this antibody was not experimentally tested to work with rat and mouse yet and we therefore cannot guarantee this.
Both antibodies have not been tested in in-cell WB and are therefore not guaranteed for it.
But I can confirm that ab416 is tested and guaranteed for human, mouse and rat. It is also tested and guaranteed for WB and ICC, which according to my information is close to the in-cell WB procedure:
In-cell WB: "A quantitative immunofluorescent assay for simultaneous analysis of two different proteins in fixed cultured cells"
I hope this information is helpful. Please do not hesitate to contact me again with any further questions.