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Western blot - EAAT1 antibody (ab416)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - EAAT1 antibody (ab416)
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Product Name
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EAAT1 antibody
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See all EAAT1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EAAT1
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Immunogen
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20 residue C-terminal synthetic peptide (Rat).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody reacts with Glutamate-aspartate transporter in CNS samples.
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-FoFr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/10 - 1/200.
ICC: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/500.
IHC-FoFr: Use at an assay dependent dilution (PMID 18714338).
WB: 1/200 - 1/5000. Predicted molecular weight: 60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; multi-pass membrane protein.
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Research areas
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Stem Cells >> Neural Stem Cells >> Surface Molecules
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Neurotransmitter >> Transporters >> Glutamate
Neuroscience >> Neurotransmitter >> Amino Acids >> Aspartate
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Relevance
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Human excitatory amino acid transporters (EAATs) are members of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. It is believed that these proteins reduce extracellular glutamate concentration, thereby modulating synaptic signaling. In addition, EAATs may also be important for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressed in the frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. EAAT1 shares some pharmacological similarities with EAAT3 and both are potent antagonists that appear to specifically block transport mediated by EAAT2.
EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport by co-transporting sodium. EAAT1 is essential for terminating the postsynaptic action of glutamate, by rapidly removing released glutamate from the synaptic cleft.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole Serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Glutamate-aspartate transporter is also known as GLAST.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAAT1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EAAT1 antibody images, references, products related to ab416 and other tools.
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EAAT1 antibody images:
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 Western blot - EAAT1 antibody (ab416)
EAAT1 antibody (ab416) + Rat brain cortex
Predicted band size : 60 kDa
Observed band size : 60 kDa
Additional bands at : 150 kDa (possible dimer).
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Immunohistochemistry (PFA perfusion fixed frozen sections) - EAAT1 antibody (ab416)
ab416 at 1/100 dilution staining EAAT1 in mouse coronal tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Adult Sprague–Dawley rats were injected intraperitoneally with pentobarbitone and were transcardially perfused-fixed with heparinized saline and 4% paraformaldehyde in PBS. Brains were removed, were postfixed in 4% paraformaldehyde for 5h, and were stored in PBS. Sections of each brain were pretreated with citrate buffer for 30 min at 65°C to increase antigen retrieval and penetration of the antibodies into the tissues. Sections were permeabilized with 1% Triton X-100 for 5 min and blocked with 3% normal horse serum in 0.1 M PBS, pH 7.4, for 60 min and incubated at room temperature for 48h with primary antibodies. An Alexa Fluor® 488 conjugated anti rabbit was used as secondary at 1/1000 dilution. Image from Barcia C. et. al., PLoS One. 2008 Aug 20;3(8):e2977 (Fig 8C).
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References for EAAT1 antibody (ab416)
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This product has been used in: (two most recent references)
Basso AS et al. Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis. J Clin Invest 118:1532-43 (2008). WB; Mouse. PubMed: 18340379
Dottori M et al. Lysophosphatidic acid inhibits neuronal differentiation of neural stem/progenitor cells derived from human embryonic stem cells. Stem Cells 26:1146-54 (2008). ICC/IF; Human. PubMed: 18308941
See all 8 publication references for this product.
If you publish research using ab416 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to EAAT1
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EAAT1 antibody - more information
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Customer reviews (feedback) regarding EAAT1 antibody |
Customer FAQs regarding EAAT1 antibody |
Protocols for EAAT1 antibody |
Price and availability of products related to EAAT1 antibody |
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EAAT1 antibody - related products:
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EAAT1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EAAT1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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