Anti-EAAT1 antibody (ab416)
Key features and details
- Rabbit polyclonal to EAAT1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EAAT1 antibody
See all EAAT1 primary antibodies -
Description
Rabbit polyclonal to EAAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
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General notes
Glutamate-aspartate transporter is also known as GLAST.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1. Lot numbers other than GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/200 - 1/5000. Predicted molecular weight: 60 kDa.
|
IHC-P | (3) |
1/50 - 1/500.
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Notes |
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WB
1/200 - 1/5000. Predicted molecular weight: 60 kDa. |
IHC-P
1/50 - 1/500. |
Target
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Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. -
Tissue specificity
Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. -
Involvement in disease
Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. -
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6507 Human
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P24942 Rat
- Unigene: 481918 Human
- Unigene: 34134 Rat
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Alternative names
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
see all
Images
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Anti-EAAT1 antibody (ab416) + Rat brain cortex
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 150 kDa (possible dimer) -
ab416 (1:500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (135)
ab416 has been referenced in 135 publications.
- You Y et al. Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain. J Extracell Vesicles 11:e12183 (2022). PubMed: 35029059
- Anastasaki C et al. Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization. STAR Protoc 3:101173 (2022). PubMed: 35199037
- Lotfi M et al. Thymoquinone Improved Nonylphenol-Induced Memory Deficit and Neurotoxicity Through Its Antioxidant and Neuroprotective Effects. Mol Neurobiol 59:3600-3616 (2022). PubMed: 35355194
- Reis L et al. Glyphosate-based herbicide induces long-lasting impairment in neuronal and glial differentiation. Environ Toxicol 37:2044-2057 (2022). PubMed: 35485992
- Lieberman R et al. Human iPSC-derived astrocytes generated from donors with globoid cell leukodystrophy display phenotypes associated with disease. PLoS One 17:e0271360 (2022). PubMed: 35921286