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ab42682
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab41751? Please let us know so that we can cite the reference in this datasheet
ab41751 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EAAT1 antibody (ab41751)
Anti-EAAT1 antibody (ab41751) at 1 µg/ml +
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - EAAT1 antibody (ab41751)
ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
EAAT1 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (41751)
EAAT1 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (41751)
EAAT1 antibody for Immunocytochemistry/ Immunofluorescence in Human (41751)
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