Anti-EAAT1 antibody (ab41751)
- Product nameAnti-EAAT1 antibodySee all EAAT1 primary antibodies ...
- DescriptionRabbit polyclonal to EAAT1
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EAAT1.
(Peptide available as ab42682.)
- Positive controlThis antibody gave a positive signal in Human Brain Tissue Lysate.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab41751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).Can be blocked with EAAT1 peptide (ab42682).|
|ICC/IF||ICC/IF: Use a concentration of 5 µg/ml.|
- FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
- Tissue specificityHighly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
- Involvement in diseaseDefects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
- Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
- Cellular localizationMembrane.
- EA6 antibodyEAA1_HUMAN antibodyEAAT1 antibody
- Excitatory amino acid transporter 1 antibodyFLJ25094 antibodyGLAST antibodyGLAST-1 antibodyGLAST1 antibodyGlial high affinity glutamate transporter antibodyHigh affinity neuronal glutamate transporter antibodyHigh-affinity neuronal glutamate transporter antibodySlc1a3 antibodySodium dependent glutamate/aspartate transporter antibodySodium-dependent glutamate/aspartate transporter 1 antibodySodium-dependent glutamate/aspartate transporter antibodySolute carrier family 1 (glial high affinity glutamate transporter) member 3 antibodySolute carrier family 1 member 3 antibody
Anti-EAAT1 antibody images
Anti-EAAT1 antibody (ab41751) at 1 µg/ml +
Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
Immunohistochemical analysis of frozen mouse dorsal cortex tissue. EAAT1 (red) was stained with ab41751 at 1/500 dilution.
References for Anti-EAAT1 antibody (ab41751)
This product has been referenced in:
- Honsa P et al. Polydendrocytes display large lineage plasticity following focal cerebral ischemia. PLoS One 7:e36816 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22590616) »
- Helms HC et al. In vitro evidence for the brain glutamate efflux hypothesis: brain endothelial cells cocultured with astrocytes display a polarized brain-to-blood transport of glutamate. Glia 60:882-93 (2012). ICC/IF . Read more (PubMed: 22392649) »