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Western blot - EAAT1 antibody (ab41751)
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Immunocytochemistry/ Immunofluorescence - EAAT1 antibody (ab41751)
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Product Name
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EAAT1 antibody
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See all EAAT1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EAAT1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EAAT1. (Peptide available as ab42682.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Cow
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).
Is unsuitable for IHC-FoFr.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Human Brain Tissue Lysate.
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Cellular localization
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Membrane; multi-pass membrane protein.
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Research areas
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Neuroscience >> Neurotransmitter >> Transporters >> Glutamate
Neuroscience >> Neurotransmitter >> Amino Acids >> Aspartate
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Relevance
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Human excitatory amino acid transporters (EAATs) are members of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. It is believed that these proteins reduce extracellular glutamate concentration, thereby modulating synaptic signaling. In addition, EAATs may also be important for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressed in the frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. EAAT1 shares some pharmacological similarities with EAAT3 and both are potent antagonists that appear to specifically block transport mediated by EAAT2.
EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport by co-transporting sodium. EAAT1 is essential for terminating the postsynaptic action of glutamate, by rapidly removing released glutamate from the synaptic cleft.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.70 - 0.70 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAAT1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EAAT1 antibody images, references, products related to ab41751 and other tools.
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EAAT1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - EAAT1 antibody (ab41751)
ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
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Search PubMed (MEDLINE) for references to EAAT1
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EAAT1 antibody - more information
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Customer reviews (feedback) regarding EAAT1 antibody |
Customer FAQs regarding EAAT1 antibody |
Protocols for EAAT1 antibody |
Price and availability of products related to EAAT1 antibody |
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EAAT1 antibody - related products:
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EAAT1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EAAT1 antibody |
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