Overview
- Product nameAnti-EAAT1 antibodySee all EAAT1 primary antibodies ...
- DescriptionRabbit polyclonal to EAAT1
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EAAT1.
(Peptide available as ab42682.)
- Positive controlThis antibody gave a positive signal in Human Brain Tissue Lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab41751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).Can be blocked with EAAT1 peptide (ab42682). |
| IHC-P | IHC-P: 1/50. |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
Target
- FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
- Tissue specificityHighly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
- Involvement in diseaseDefects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
- Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
- Post-translational
modificationsGlycosylated. - Cellular localizationMembrane.
-
Database links
- Entrez Gene: 282354 Cow
- Entrez Gene: 6507 Human
- Entrez Gene: 20512 Mouse
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P46411 Cow
- SwissProt: P43003 Human
- SwissProt: P56564 Mouse
- SwissProt: P24942 Rat
- Unigene: 481918 Human
- Unigene: 204834 Mouse
- Unigene: 34134 Rat
see all
Target information above from: UniProt accession
P43003
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- EA6 antibodyEAA1_HUMAN antibodyEAAT1 antibody
- Excitatory amino acid transporter 1 antibodyFLJ25094 antibodyGLAST antibodyGLAST-1 antibodyGLAST1 antibodyGlial high affinity glutamate transporter antibodyHigh affinity neuronal glutamate transporter antibodyHigh-affinity neuronal glutamate transporter antibodySlc1a3 antibodySodium dependent glutamate/aspartate transporter antibodySodium-dependent glutamate/aspartate transporter 1 antibodySodium-dependent glutamate/aspartate transporter antibodySolute carrier family 1 (glial high affinity glutamate transporter) member 3 antibodySolute carrier family 1 member 3 antibody
see all
Anti-EAAT1 antibody images
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Western blot - EAAT1 antibody (ab41751)Anti-EAAT1 antibody (ab41751) at 1 µg/ml +Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - EAAT1 antibody (ab41751)ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml. -
Immunohistochemistry (Frozen sections) - Anti-EAAT1 antibody (ab41751)Image from Honsa P et al., PLoS One. 2012;7(5):e36816. Epub 2012 May 10. Fig 3.; doi:10.1371/journal.pone.0036816; May 10, 2012, PLoS ONE 7(5): e36816.Immunohistochemical analysis of frozen mouse dorsal cortex tissue. EAAT1 (red) was stained with ab41751 at 1/500 dilution.
References for Anti-EAAT1 antibody (ab41751)
This product has been referenced in:
- Honsa P et al. Polydendrocytes display large lineage plasticity following focal cerebral ischemia. PLoS One 7:e36816 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22590616) »
- Helms HC et al. In vitro evidence for the brain glutamate efflux hypothesis: brain endothelial cells cocultured with astrocytes display a polarized brain-to-blood transport of glutamate. Glia 60:882-93 (2012). ICC/IF . Read more (PubMed: 22392649) »
