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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-EAAT1 antibody
See all EAAT1 products (5) ...
Description
Rabbit polyclonal to EAAT1
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EAAT1.
(Peptide available as ab42682.)
Positive control
This antibody gave a positive signal in Human Brain Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmitter >> Transporters >> Glutamate
Neuroscience >> Neurotransmitter >> Amino Acids >> Aspartate
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 56 kDa (predicted molecular weight: 60 kDa).
IHC-P: 1/50
ICC/IF: Use a concentration of 5 µg/ml
Application notes
Is unsuitable for or IHC-FoFr.
Target
Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Tissue specificity
Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Involvement in disease
Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionP43003
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
see all
Database links
- Entrez Gene: 282354 Cow
- Entrez Gene: 6507 Human
- Entrez Gene: 20512 Mouse
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P46411 Cow
- SwissProt: Q0VCK3 Cow
- SwissProt: P43003 Human
see all
Anti-EAAT1 antibody images:
Western blot - EAAT1 antibody (ab41751)
Anti-EAAT1 antibody (ab41751) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 56 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - EAAT1 antibody (ab41751)
ICC/IF image of ab41751 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41751, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
References for Anti-EAAT1 antibody (ab41751)
ab41751 has not yet been referenced specifically in any publications.
Publishing research using ab41751? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"