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Anti-EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)

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Overview

Product name

Anti-EAAT2 antibody [10B7] - Carboxyterminal end
See all EAAT2 products (10) ...

Description

Mouse monoclonal [10B7] to EAAT2 - Carboxyterminal end

Specificity

This antibody is specific for EAAT2. It does not react with GLT1a.

Tested applications

IHC-P, WB, ICCmore details

Cross reactivity

Reacts with

Rat

Predicted to work with

Mouse

Does not react with

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of rat EAAT2.

Positive control

Transfected COS7 lysate and rat brain.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS, Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

10B7

Isotype

IgG2a

Research areas

Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate

  • Western blot - EAAT2 antibody [10B7] (ab61859)Western blot - EAAT2 antibody [10B7] (ab61859) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859)Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab61859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent dilution.

  • ShowHide1 Image

    WB

     WB: 1/200 - 1/500.Detect...Read more →

    WB: 1/200 - 1/500.Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).

  • ICC

     ICC: 1/200 - 1/500.

    ICC: 1/200 - 1/500.

Target

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.

Post-translational
modifications

Glycosylated.

Cellular localization

Membrane.

Target information above from: UniProt accessionP43004 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • EAA2_HUMAN antibody
  • EAAT2 antibody
  • Excitatory amino acid transporter 2 antibody
  • Excitotoxic amino acid transporter 2 antibody
  • Glial high affinity glutamate transporter antibody
  • GLT 1 antibody
  • GLT1 antibody
  • Glutamate aspartate transporter II antibody
  • Glutamate/aspartate transporter II antibody
  • SLC1A2 antibody
  • Sodium dependent glutamate aspartate transporter 2 antibody
  • Sodium dependent glutamate/aspartate transporter 2 antibody
  • Sodium-dependent glutamate/aspartate transporter 2 antibody
  • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
  • Solute carrier family 1 member 2 antibody
see all

Database links

Anti-EAAT2 antibody [10B7] - Carboxyterminal end images:

  Western blot - EAAT2 antibody [10B7] (ab61859)

Western blot - EAAT2 antibody [10B7] (ab61859)

All lanes : Anti-EAAT2 antibody [10B7] - Carboxyterminal end (ab61859) at 1/200 dilution

Lane 1 : COS7 cells transfected with GLT1a
Lane 2 : COS7 cells transfected with EAAT2


Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

  Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859)

Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859)

Immunocytochemistry on COS7 cells transfected with EAAT2 using ab61859 (1:200).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)

Ab61859 (1:200) staining rat EAAT2 in rat brain by immunofluorescence. EAAT2 is shown in green and the main GLT1 protein is shown in red.

References for Anti-EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)

This product has been referenced in:

  • Liu AYet al. Neuroprotective Drug Riluzole Amplifies the Heat Shock Factor 1 (HSF1)- and Glutamate Transporter 1 (GLT1)-dependent Cytoprotective Mechanisms for Neuronal Survival. J Biol Chem 286:2785-94 (2011). WB; Rat.Read more (PubMed: 21098017) »
  • Rose EMet al. Glutamate transporter coupling to Na,K-ATPase. J Neurosci 29:8143-55 (2009). IHC-Fr; Rat.Read more (PubMed: 19553454) »

See all 2 publications for this product

Publishing research using ab61859? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"