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Western blot - EAAT2 antibody [10B7] (ab61859)
![Western blot - EAAT2 antibody [10B7] (ab61859)](/ps/datasheet/images/ab61859_1.jpg)
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Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859)
![Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859)](/ps/datasheet/images/ab61859_2.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)](/ps/datasheet/images/ab61859_3.jpg)
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Product Name
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EAAT2 antibody [10B7] - Carboxyterminal end
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See all EAAT2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [10B7] to EAAT2 - Carboxyterminal end
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Immunogen
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A synthetic peptide corresponding to a C-terminal region of rat EAAT2.
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Reacts with
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Rat
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Does not react with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Specificity
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This antibody is specific for EAAT2. It does not react with GLT1a.
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Tested applications
(see key)
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ICC, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/200 - 1/500.
IHC-P: Use at an assay dependent dilution.
WB: 1/200 - 1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Transfected COS7 lysate and rat brain.
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Cellular localization
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Membrane; Multi-pass membrane protein
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Research areas
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Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Relevance
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EAAT2 is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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10B7
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Isotype
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IgG2a
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Purity
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Ascites
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Storage buffer
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Preservative: None
Constituents: PBS, Ascites
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Form
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Liquid
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Concentration
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0.600 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAAT2 antibody [10B7] - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EAAT2 antibody [10B7] - Carboxyterminal end images, references, products related to ab61859 and other tools.
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EAAT2 antibody [10B7] - Carboxyterminal end images:
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 Western blot - EAAT2 antibody [10B7] (ab61859)
All lanes : EAAT2 antibody [10B7] - Carboxyterminal end (ab61859) at 1/200 dilution
Lane 1 : COS7 cells transfected with GLT1a
Lane 2 : COS7 cells transfected with EAAT2
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859)
Immunocytochemistry on COS7 cells transfected with EAAT2 using ab61859 (1:200).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)
Ab61859 (1:200) staining rat EAAT2 in rat brain by immunofluorescence. EAAT2 is shown in green and the main GLT1 protein is shown in red.
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References for EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)
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This product has been used in:
Rose EM et al. Glutamate transporter coupling to Na,K-ATPase. J Neurosci 29:8143-55 (2009). IHC-Fr; Rat. PubMed: 19553454
If you publish research using ab61859 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to EAAT2
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EAAT2 antibody [10B7] - Carboxyterminal end - more information
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Customer reviews (feedback) regarding EAAT2 antibody [10B7] - Carboxyterminal end |
Customer FAQs regarding EAAT2 antibody [10B7] - Carboxyterminal end |
Protocols for EAAT2 antibody [10B7] - Carboxyterminal end |
Price and availability of products related to EAAT2 antibody [10B7] - Carboxyterminal end |
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EAAT2 antibody [10B7] - Carboxyterminal end - related products:
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EAAT2 antibody [10B7] - Carboxyterminal end - other tools:
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Contact Abcam with a Technical Enquiry about EAAT2 antibody [10B7] - Carboxyterminal end |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - EAAT2 antibody [10B7] (ab61859)](/index.html?datasheet=61859#ab61859_1.jpg)
![Immunocytochemistry/ Immunofluorescence - EAAT2 antibody [10B7] (ab61859)](/index.html?datasheet=61859#ab61859_2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [10B7] - Carboxyterminal end (ab61859)](/index.html?datasheet=61859#ab61859_3.jpg)
