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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645)](/ps/datasheet/images/ab49645_1.JPG)
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Product Name
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EAAT2 antibody [1H8] - Carboxyterminal end
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See all EAAT2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1H8] to EAAT2 - Carboxyterminal end
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Immunogen
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recombinant protein corresponding to the C-terminal, cytoplasmic domain of human EAAT2.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: 1/20 - 1/40.
IHC-P: 1/20 - 1/40.
Note: High temperature antigen unmasking technique recommended.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Immunohistochemistry - Human brain, motor cortex region
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Cellular localization
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Membrane; Multi-pass membrane protein
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Research areas
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Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Relevance
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EAAT2 is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1H8
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Myeloma
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P3-NS1/1-Ag4-1
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Isotype
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IgG2a
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAAT2 antibody [1H8] - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EAAT2 antibody [1H8] - Carboxyterminal end images, references, products related to ab49645 and other tools.
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EAAT2 antibody [1H8] - Carboxyterminal end images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645)
Human brain, normal adult cerebellum: IHC-P staining for excitatory amino acid transporter 2 using ab49645. Note membrane staining of glial cells.
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Search PubMed (MEDLINE) for references to EAAT2
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EAAT2 antibody [1H8] - Carboxyterminal end - more information
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Protocols for EAAT2 antibody [1H8] - Carboxyterminal end |
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EAAT2 antibody [1H8] - Carboxyterminal end - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645)](/index.html?datasheet=49645#ab49645_1.JPG)
