![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645) image](#ab49645_1.JPG)
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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-EAAT2 antibody [1H8] - Carboxyterminal end
See all EAAT2 products (10) ...
Description
Mouse monoclonal [1H8] to EAAT2 - Carboxyterminal end
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
recombinant protein corresponding to the C-terminal, cytoplasmic domain of human EAAT2.
Positive control
Immunohistochemistry - Human brain, motor cortex region
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
1H8
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG2a
Research areas
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645) image](/ps/datasheet/Images/49/ab49645/ab49645_1.JPG)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
IHC-Fr: 1/20 - 1/40.IHC-Fr: 1/20 - 1/40.
Application notes
Is unsuitable for or WB.
Target
Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionP43004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EAA2_HUMAN antibody
- EAAT2 antibody
- Excitatory amino acid transporter 2 antibody
see all
Database links
- Entrez Gene: 6506 Human
- Omim: 600300 Human
- SwissProt: P43004 Human
- Unigene: 502338 Human
Anti-EAAT2 antibody [1H8] - Carboxyterminal end images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EAAT2 antibody [1H8] - Carboxyterminal end (ab49645)
Human brain, normal adult cerebellum: IHC-P staining for excitatory amino acid transporter 2 using ab49645. Note membrane staining of glial cells.
References for Anti-EAAT2 antibody [1H8] - Carboxyterminal end (ab49645)
ab49645 has not yet been referenced specifically in any publications.
Publishing research using ab49645? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"