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Western blot - EAAT2 antibody (ab54417)
(enlarge)
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Product Name
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EAAT2 antibody
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See all EAAT2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EAAT2
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Immunogen
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Synthetic peptide: VRMHDSHLSSEEPKHR conjugated to KLH, corresponding to internal sequence amino acids 16-31 of Rat EAAT2
BLAST 'VRMHDSHLSSEEPKHR' with  or 
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu, Cat, Chmp, CynMk, Dog, RMk, Orngt
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 62 kDa.
Is unsuitable for IHC-Fr.
Is unsuitable for IHC on 4% paraformaldehyde fixed mouse or rat brain tissue.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse brain extract. Rat brain extract.
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Cellular localization
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Membrane; Multi-pass membrane protein
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Research areas
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Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Relevance
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EAAT2 is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors.
A protein database search confirms that the immunogen sequence is unique to the Excitatory Amino Acid Transporter (EAAT2) protein. By immunoblotting, ab54417 detects native protein migrating at approximately 50 kDa in SDS-PAGE blots using total brain extract from mouse or rat. This signal was completely abolished by preincubating the purified antibody with a control immunogen peptide at 25 µg/ml.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate
ab54417 is supplied with an unconjugated Excitatory Amino Acid Transporter (EAAT2) Control Peptide for use as an internal control for antibody specificity. Reconstitute peptide with 25 µl dH2O for a working stock of 1 mg/ml. To use preincubate the purified antibody with peptide at 25 µg/ml. Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAAT2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for EAAT2 antibody images, references, products related to ab54417 and other tools.
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EAAT2 antibody images:
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 Western blot - EAAT2 antibody (ab54417)
All lanes : EAAT2 antibody (ab54417) at 2 µg/ml
Lane 1 : Whole tissue extract from mouse brain
Lane 2 : Whole tissue extract from mouse brain with control peptide
Lysates/proteins at 100 µg per lane.
developed using the ECL technique
Predicted band size : 62 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to EAAT2
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EAAT2 antibody - more information
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Customer reviews (feedback) regarding EAAT2 antibody |
Customer FAQs regarding EAAT2 antibody |
Protocols for EAAT2 antibody |
Price and availability of products related to EAAT2 antibody |
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EAAT2 antibody - related products:
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EAAT2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about EAAT2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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