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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-EAAT2 antibody
See all EAAT2 products (10) ...
Description
Rabbit polyclonal to EAAT2
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Horse, Cow, Cat, Dog, Human, Chimpanzee, Cynomolgus Monkey, Rhesus monkey, Opossum, Orangutan
Immunogen
Synthetic peptide: VRMHDSHLSSEEPKHR conjugated to KLH, corresponding to internal sequence amino acids 16-31 of Rat EAAT2
VRMHDSHLSS EEPKHR
Positive control
Mouse brain extract. Rat brain extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate
ab54417 is supplied with an unconjugated Excitatory Amino Acid Transporter (EAAT2) Control Peptide for use as an internal control for antibody specificity. Reconstitute peptide with 25 µl dH2O for a working stock of 1 mg/ml. To use preincubate the purified antibody with peptide at 25 µg/ml.
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
A protein database search confirms that the immunogen sequence is unique to the Excitatory Amino Acid Transporter (EAAT2) protein. By immunoblotting, ab54417 detects native protein migrating at approximately 50 kDa in SDS-PAGE blots using total brain extract from mouse or rat. This signal was completely abolished by preincubating the purified antibody with a control immunogen peptide at 25 µg/ml.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Western blot - EAAT2 antibody (ab54417)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 62 kDa.
Is unsuitable for IHC-Fr.
Is unsuitable for IHC on 4% paraformaldehyde fixed mouse or rat brain tissue.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionP43004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EAA2_HUMAN antibody
- EAAT2 antibody
- Excitatory amino acid transporter 2 antibody
see all
Database links
- Entrez Gene: 6506 Human
- Entrez Gene: 20511 Mouse
- Entrez Gene: 29482 Rat
- Omim: 600300 Human
- SwissProt: P43004 Human
- SwissProt: P43006 Mouse
- SwissProt: P31596 Rat
- Unigene: 502338 Human
see all
Anti-EAAT2 antibody images:
Western blot - EAAT2 antibody (ab54417)
All lanes : Anti-EAAT2 antibody (ab54417) at 2 µg/ml
Lane 1 : Whole tissue extract from mouse brain
Lane 2 : Whole tissue extract from mouse brain with control peptide
Lysates/proteins at 100 µg per lane.
developed using the ECL technique
Predicted band size : 62 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-EAAT2 antibody (ab54417)
ab54417 has not yet been referenced specifically in any publications.
Publishing research using ab54417? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"