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Western blot - EAAT2 antibody (ab54417)
Western blot - EAAT2 antibody (ab54417)
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Product Name 

EAAT2 antibody

 

See all EAAT2 antibodies (10)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EAAT2

Immunogen 

Synthetic peptide: VRMHDSHLSSEEPKHR conjugated to KLH, corresponding to internal sequence amino acids 16-31 of Rat EAAT2
BLAST 'VRMHDSHLSSEEPKHR' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Ms, Rat

Predicted to react 
(species key)

Hu, Cat, Chmp, CynMk, Dog, RMk, Orngt
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 62 kDa.

Is unsuitable for IHC-Fr.
Is unsuitable for IHC on 4% paraformaldehyde fixed mouse or rat brain tissue.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse brain extract. Rat brain extract.

Cellular localization 

Membrane; Multi-pass membrane protein

Research areas 

Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate

Relevance 

EAAT2 is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors.

A protein database search confirms that the immunogen sequence is unique to the Excitatory Amino Acid Transporter (EAAT2) protein. By immunoblotting, ab54417 detects native protein migrating at approximately 50 kDa in SDS-PAGE blots using total brain extract from mouse or rat. This signal was completely abolished by preincubating the purified antibody with a control immunogen peptide at 25 µg/ml.

 

Alternative names EAAT2 antibody (ab54417)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

6506    (Human)
20511    (Mouse)
29482    (Rat)

Omim

    

600300    (Human)

SwissProt

    

P43004    (Human)
P43006    (Mouse)
P31596    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate

ab54417 is supplied with an unconjugated Excitatory Amino Acid Transporter (EAAT2) Control Peptide for use as an internal control for antibody specificity. Reconstitute peptide with 25 µl dH2O for a working stock of 1 mg/ml. To use preincubate the purified antibody with peptide at 25 µg/ml.

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAAT2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for EAAT2 antibody images, references, products related to ab54417 and other tools.

 

EAAT2 antibody images:

Product image  Western blot - EAAT2 antibody (ab54417)

Western blot - EAAT2 antibody (ab54417)

All lanes : EAAT2 antibody (ab54417) at 2 µg/ml

Lane 1 : Whole tissue extract from mouse brain
Lane 2 : Whole tissue extract from mouse brain with control peptide

Lysates/proteins at 100 µg per lane.

developed using the ECL technique

Predicted band size : 62 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to EAAT2

 

EAAT2 antibody - more information

Customer reviews (feedback) regarding EAAT2 antibody

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Price and availability of products related to EAAT2 antibody

 

EAAT2 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for EAAT2 antibody

ab29473

  

Brain (Rat) Membrane Lysate - normal tissue

ab29475

  

Brain (Rat) Whole Cell Lysate - normal tissue

ab30149

  

Brain (Mouse) Membrane Lysate - normal tissue

ab30151

  

Brain (Mouse) Whole Cell Lysate - normal tissue

 

Compatible Secondaries for EAAT2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other EAAT2 Antibodies

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EAAT2 antibody - other tools:

Contact Abcam with a Technical Enquiry about EAAT2 antibody

 

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