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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-EAAT2 antibody
See all EAAT2 products (10) ...
Description
Rabbit polyclonal to EAAT2
Specificity
This antibody recognizes the ~75 kDa and the ~50 kDa forms of EAAT2. This antibody may also cross react non-specifically with a ~102 kDa protein not believed to be related to EAAT2.
Tested applications
ICC/IF, WB, IPmore details
Cross reactivity
Reacts with
Rat
Predicted to work with
Mouse, Cat, Dog, Human, Chimpanzee, Rhesus monkey, Orangutan
Immunogen
Synthetic peptide: NGKSADCSVEEEPWKREK) (Rat), corresponding to amino acids at the C-terminus of EAAT2.
NGKSADCSVE EEPWKREK)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purification notes
Supplied as Serum diluted in PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Immunocytochemistry/ Immunofluorescence - Anti-EAAT2 antibody (ab58571)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab58571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200
WB: 1/1000Predicted molecular weight: 62 kDa.
IP: Use at an assay dependent dilution.
Target
Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
Post-translational
modifications
Glycosylated.
Cellular localization
Membrane.
Target information above from: UniProt accessionP43004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EAA2_HUMAN antibody
- EAAT2 antibody
- Excitatory amino acid transporter 2 antibody
see all
Database links
- Entrez Gene: 6506 Human
- Entrez Gene: 20511 Mouse
- Entrez Gene: 29482 Rat
- Omim: 600300 Human
- SwissProt: P43004 Human
- SwissProt: P43006 Mouse
- SwissProt: P31596 Rat
- Unigene: 502338 Human
see all
Anti-EAAT2 antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-EAAT2 antibody (ab58571)
ICC/IF image of ab58571 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58571, 1:200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-EAAT2 antibody (ab58571)
This product has been referenced in:
- Sama MAet al. Interleukin-1{beta}-dependent Signaling between Astrocytes and Neurons Depends Critically on Astrocytic Calcineurin/NFAT Activity. J Biol Chem 283:21953-64 (2008). WB; Rat.Read more (PubMed: 18541537) »
See 1 publication for this product
Publishing research using ab58571? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"