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Products:Cell Biology >> Apoptosis >> Nucleus >> Other
Overview
Product name
Anti-EAF2 antibody
Description
Rabbit polyclonal to EAF2
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: AGFSHLDRRERV, corresponding to amino acids 5-16 of Human EAF2.
AGFSHLDRRE RV
Positive control
Rat and mouse liver for Western blot.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline. pH 7.4.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Apoptosis >> Nucleus >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Application notes
IHC-P: Use at a concentration of 2.5 µg/ml.
WB: Use at a concentration of 1.5 µg/ml. Predicted molecular weight: 29 kDa. This protein migrates to around 43kDa when a western blot is performed. We have found that the same happened in other literature as well. A paper published by Federico Simone shows that this protein can migrate to 43kDa (see references).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
Tissue specificity
Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
Sequence similarities
Belongs to the EAF family.
Cellular localization
Nucleus speckle. Colocalizes with ELL to nuclear speckles.
Target information above from: UniProt accessionQ96CJ1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BM 040 antibody
- BM040 antibody
- EAF 2 antibody
see all
Database links
- Entrez Gene: 55840 Human
- Entrez Gene: 106389 Mouse
- Entrez Gene: 266787 Rat
- SwissProt: Q96CJ1 Human
- SwissProt: Q91ZD6 Mouse
- SwissProt: Q811X5 Rat
- Unigene: 477325 Human
- Unigene: 432078 Mouse
see all
References for Anti-EAF2 antibody (ab28357)
ab28357 has not yet been referenced specifically in any publications.
Publishing research using ab28357? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"