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Product Name
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EAF2 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EAF2
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Immunogen
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Synthetic peptide: AGFSHLDRRERV, corresponding to amino acids 5-16 of Human EAF2.
BLAST 'AGFSHLDRRERV' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 2.5 µg/ml.
WB: Use at a concentration of 1.5 µg/ml. Predicted molecular weight: 29 kDa. This protein migrates to around 43kDa when a western blot is performed. We have found that the same happened in other literature as well. A paper published by Federico Simone shows that this protein can migrate to 43kDa (see references).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat and mouse liver for Western blot.
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Cellular localization
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Nuclear. Colocalizes with ELL to nuclear speckles.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Apoptosis >> Nucleus >> Other
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Relevance
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EAF2 is a testosterone regulated apoptosis inducer with tumor suppressive activity. In vivo, overexpression of U19 induces massive apoptosis and inhibits prostate tumor growth. EAF2 mRNA is widely expressed in various tissues, with the most abundant expression in the prostate, kidney, bone marrow and lymph nodes.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 55840 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline. pH 7.4. Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EAF2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to EAF2
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EAF2 antibody - more information
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