Recombinant Anti-EB3 antibody [EPR11421(B)] (ab157217)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11421(B)] to EB3
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-EB3 antibody [EPR11421(B)]
See all EB3 primary antibodies -
Description
Rabbit monoclonal [EPR11421(B)] to EB3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human EB3. The exact sequence is proprietary.
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Positive control
- WB: Human cerebellum, human fetal brain and rat muscle lysates. IHC-P: Human brain tissue, Human skeletal muscle tissue. Flow Cyt (intra): U87 MG cells. ICC/IF: SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11421(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 32 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Predominantly expressed in brain and muscle. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Associated with the microtubule network. - Information by UniProt
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Database links
- Entrez Gene: 22924 Human
- Entrez Gene: 100732 Mouse
- Entrez Gene: 298848 Rat
- Omim: 605788 Human
- SwissProt: Q9UPY8 Human
- SwissProt: Q6PER3 Mouse
- SwissProt: Q5XIT1 Rat
- Unigene: 515860 Human
see all -
Alternative names
- APC binding protein antibody
- EB 3 antibody
- EB1 protein family member 3 antibody
see all
Images
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All lanes : Anti-EB3 antibody [EPR11421(B)] (ab157217) at 1/10000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Rat muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa -
Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling EB3 with purified ab157217 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control: PBS only.
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Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labeling EB3 with ab157217 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of U87-MG (human glioblastoma) cells labeling EB3 with unpurified ab157217 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemical analysis of paraffin embedded human brain tissue labeling EB3 with ab157217 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab157217 has been referenced in 8 publications.
- Kauer SD et al. Inositol Polyphosphate-5-Phosphatase K (Inpp5k) Enhances Sprouting of Corticospinal Tract Axons after CNS Trauma. J Neurosci 42:2190-2204 (2022). PubMed: 35135857
- Pchitskaya E et al. Cytoskeleton Protein EB3 Contributes to Dendritic Spines Enlargement and Enhances Their Resilience to Toxic Effects of Beta-Amyloid. Int J Mol Sci 23:N/A (2022). PubMed: 35216391
- Du M et al. Direct interaction of metastasis-inducing S100P protein with tubulin causes enhanced cell migration without changes in cell adhesion. Biochem J 477:1159-1178 (2020). PubMed: 32065231
- Sayas CL et al. Role of tau N-terminal motif in the secretion of human tau by End Binding proteins. PLoS One 14:e0210864 (2019). PubMed: 30668577
- Calipari ES et al. Synaptic Microtubule-Associated Protein EB3 and SRC Phosphorylation Mediate Structural and Behavioral Adaptations During Withdrawal From Cocaine Self-Administration. J Neurosci 39:5634-5646 (2019). PubMed: 31092585
- Xie B et al. Poly(ADP-ribose) mediates asymmetric division of mouse oocyte. Cell Res 28:462-475 (2018). PubMed: 29463901
- He B et al. Neuroprotective effect of lurasidone via antagonist activities on histamine in a rat model of cranial nerve involvement. Mol Med Rep 17:6002-6008 (2018). PubMed: 29436643
- Berges R et al. End-binding 1 protein overexpression correlates with glioblastoma progression and sensitizes to Vinca-alkaloids in vitro and in vivo. Oncotarget 5:12769-87 (2014). PubMed: 25473893