You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-EBAG9 antibody [ZR001] - Carboxyterminal end (ab54241)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-EBAG9 antibody [ZR001] - Carboxyterminal end
See all EBAG9 products (7) ...

Description

Mouse monoclonal [ZR001] to EBAG9 - Carboxyterminal end

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Recombinant fragment (Human) derived from the C-terminal region of the EBAG9.

Positive control

Human MCF-7 cell lysate and Human 293 cell lysate (Western blotting) and Human colon cancer tissue (Immunohistochemistry).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

ZR001

Isotype

IgG1

Light chain type

kappa

Research areas

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Growth Factors/Hormones >> Hormones
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

  • Western blot - EBAG9 antibody [ZR001] (ab54241)Western blot - EBAG9 antibody [ZR001] (ab54241) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab54241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IHC-P: Use at a concentration of 5 - 10 µg/ml. Heat Induced Epitope Retrieval (HIER) with citrate buffer is required for immunohistochemistry of paraffin-embedded tissues.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 24 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

EBAG9 is a type III transmembrane protein that is predominantly located in the Golgi. It induces apoptosis of natural killer (NK)/T cells and plays a role in immune invasion, possibly in relation to Epstein-Bar virus infection. EBAG9 is abundantly expressed in prostate cancer cells when compared to normal epithelial cells, and immunodetection of EBAG9 expression can be a negative prognostic indicator for patients with prostate cancer.

Cellular localization

Golgi apparatus; Golgi apparatus membrane; single pass type III membrane protein.

Alternative names

  • BAG9 antibody
  • Cancer associated surface antigen antibody
  • Cancer associated surface antigen RCAS1 antibody
  • EB9 antibody
  • EBAG 9 antibody
  • Estrogen receptor binding fragment associated gene 9 protein antibody
  • Estrogen receptor binding site associated antigen 9 antibody
  • PDAF antibody
  • RCAS 1 antibody
  • RCAS1 antibody
  • Receptor binding cancer antigen expressed on SiSo cells antibody
see all

Database links

see all

Anti-EBAG9 antibody [ZR001] - Carboxyterminal end images:

  Western blot - EBAG9 antibody [ZR001] (ab54241)

Western blot - EBAG9 antibody [ZR001] (ab54241)

Anti-EBAG9 antibody [ZR001] - Carboxyterminal end (ab54241) at 1 µg/ml + MCF-7 cell lysate

Predicted band size : 24 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References for Anti-EBAG9 antibody [ZR001] - Carboxyterminal end (ab54241)

ab54241 has not yet been referenced specifically in any publications.

Publishing research using ab54241? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"