EBAG9 antibody [ZR001] - Carboxyterminal end (ab54241)

Product Name 

EBAG9 antibody [ZR001] - Carboxyterminal end

Product type 

Primary antibodies

Description 

Mouse monoclonal [ZR001] to EBAG9 - Carboxyterminal end

Immunogen 

Recombinant fragment (Human) derived from the C-terminal region of the EBAG9.

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IHC-P: Use at a concentration of 5 - 10 µg/ml. Heat Induced Epitope Retrieval (HIER) with citrate buffer is required for immunohistochemistry of paraffin-embedded tissues.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 24 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human MCF-7 cell lysate and Human 293 cell lysate (Western blotting) and Human colon cancer tissue (Immunohistochemistry).

Cellular localization 

Golgi apparatus; Golgi apparatus membrane; single pass type III membrane protein.

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Growth Factors/Hormones >> Hormones
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

EBAG9 is a type III transmembrane protein that is predominantly located in the Golgi. It induces apoptosis of natural killer (NK)/T cells and plays a role in immune invasion, possibly in relation to Epstein-Bar virus infection. EBAG9 is abundantly expressed in prostate cancer cells when compared to normal epithelial cells, and immunodetection of EBAG9 expression can be a negative prognostic indicator for patients with prostate cancer.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ZR001

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: Tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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